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 <!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.0 20120330//EN" "http://jats.nlm.nih.gov/publishing/1.0/JATS-journalpublishing1.dtd"> <article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" article-type="case-report" dtd-version="1.0" xml:lang="en">
  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">JAR</journal-id>
      <journal-title-group>
        <journal-title>Journal of Agronomy Research</journal-title>
      </journal-title-group>
      <issn pub-type="epub">2639-3166</issn>
      <publisher>
        <publisher-name>Open Access Pub</publisher-name>
        <publisher-loc>United States</publisher-loc>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="publisher-id">JAR-18-2239</article-id>
      <article-id pub-id-type="doi">10.14302/issn.2639-3166.jar-18-2239</article-id>
      <article-categories>
        <subj-group>
          <subject>case-report</subject>
        </subj-group>
      </article-categories>
      <title-group>
        <article-title>Effect of Nitrogen Fertilizer on some Attributes of SorghumGrown in Saline Soil under Irrigation </article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author">
          <name>
            <surname>Abubaker</surname>
            <given-names>Haroun M.Adam</given-names>
          </name>
          <xref ref-type="aff" rid="idm1842941436">1</xref>
          <xref ref-type="corresp" rid="cor1">*</xref>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Mohammed</surname>
            <given-names>Ahmed Taleim</given-names>
          </name>
          <xref ref-type="aff" rid="idm1842941436">1</xref>
        </contrib>
      </contrib-group>
      <aff id="idm1842941436">
        <label>1</label>
        <addr-line>Department of Crop Science, College of Agriculture, University of Bahri-Sudan.</addr-line>
      </aff>
      <contrib-group>
        <contrib contrib-type="editor">
          <name>
            <surname>Berken</surname>
            <given-names>Cimen</given-names>
          </name>
          <xref ref-type="aff" rid="idm1843056612">1</xref>
        </contrib>
      </contrib-group>
      <aff id="idm1843056612">
        <label>1</label>
        <addr-line>Cukurova University, Turkey.</addr-line>
      </aff>
      <author-notes>
        <corresp id="cor1">Correspondence: Abubaker Haroun M. Adam, Department of Crop Science, College of Agriculture, University of Bahri, Sudan; Email: <email>E.abubakerharoun@gmail.com</email>.</corresp>
        <fn fn-type="conflict" id="idm1850392284">
          <p>The authors have declared that no competing interests exist.</p>
        </fn>
      </author-notes>
      <pub-date pub-type="epub" iso-8601-date="2018-09-11">
        <day>11</day>
        <month>09</month>
        <year>2018</year>
      </pub-date>
      <volume>1</volume>
      <issue>2</issue>
      <fpage>12</fpage>
      <lpage>17</lpage>
      <history>
        <date date-type="received">
          <day>22</day>
          <month>07</month>
          <year>2018</year>
        </date>
        <date date-type="accepted">
          <day>28</day>
          <month>08</month>
          <year>2018</year>
        </date>
        <date date-type="online">
          <day>11</day>
          <month>09</month>
          <year>2018</year>
        </date>
      </history>
      <permissions>
        <copyright-statement/>
        <copyright-year>2018</copyright-year>
        <copyright-holder>Abubaker Haroun M.Adam,et al.</copyright-holder>
        <license xlink:href="http://creativecommons.org/licenses/by/4.0/" xlink:type="simple">
          <license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p>
        </license>
      </permissions>
      <self-uri xlink:href="http://openaccesspub.org/jar/article/828">This article is available from http://openaccesspub.org/jar/article/828</self-uri>
      <abstract>
        <p>The main objective of this study was to investigate the effects of 4 levels of Nitrogen (N) fertilizer (0, 25, 50 and 75 Kg N/fed) on some attributes of Sorghum (<italic>Sorghum bicolor</italic> L). This study was conducted in the farm of College of Agriculture, University of Bahri-Sudan; during the period 2014-2015. Where the soil is classified as saline with pH ranging from 7.5 -7.8. A randomized completely block designed (RCBD); with 3 replications and four (4) treatments was adopted. All recommended cultural practices were carried out timely. Data; pertaining some plant attributes were collected and analyzed. The result showed that the application of 75KgN/fed has increased both; the average number of leaves as well as the plant height compared to other levels of Nitrogen fertilizer including the control (<xref ref-type="fig" rid="idm1842643396">Figure 1</xref>, <xref ref-type="fig" rid="idm1842641740">Figure 2</xref>). For the average number of tillers concern, there is non -significant difference between all Nitrogen levels and average number of tillers (<xref ref-type="fig" rid="idm1842639292">Figure 3</xref>). The study concluded that the Nitrogen fertilizer has variable effects on the studied agronomic characters, and the application of 75kgN/fed is the optimal level of fertilizer for improving the agronomic traits of Sorghum under Alkadaro conditions.</p>
      </abstract>
      <kwd-group>
        <kwd>Germination</kwd>
        <kwd>fertilizer</kwd>
        <kwd>Kadaro</kwd>
        <kwd>Parameter</kwd>
      </kwd-group>
      <counts>
        <fig-count count="4"/>
        <table-count count="0"/>
        <page-count count="6"/>
      </counts>
    </article-meta>
  </front>
  <body>
    <sec id="idm1842784068" sec-type="intro">
      <title>Introduction</title>
      <p>Sorghum (<italic>Sorghum bicolor L. </italic><italic>Moench</italic>) is an annual self- pollinated crop which belongs to family gramineae (poaecae).  It ranks the fifth most important cereal crops in the world. It is grown in the arid,               semi-arid, tropical and sub-tropical regions of the world between 4<sup>0</sup> N and 4<sup>0</sup> S of equator <xref ref-type="bibr" rid="ridm1842834300">1</xref>. </p>
      <p>This crop is sensitive to low temperature, but tolerates high temperature better than any other cereal crops. Therefore; it is called drought tolerant. Nevertheless, it can be produced on wide range of soil (pH) ranging from 5.0 to 8.5 <xref ref-type="bibr" rid="ridm1842838044">2</xref>, but the production is influenced by various factors, among these, the moisture, temperature, soil type, irrigation water, fertilizers, and soil pH <xref ref-type="bibr" rid="ridm1842916172">3</xref>.</p>
      <p>Sorghum is stable food for millions of people in Africa and India. It is widely used for human consumption; as well as animal feed. The dry stalks are commonly used as fuel for cooking and fencing or roofing huts<xref ref-type="bibr" rid="ridm1842934564">4</xref>.  The Sorghum grain also used in manufacturing starch, alcohol and glucose <xref ref-type="bibr" rid="ridm1842691068">5</xref>.</p>
      <p>Sorghum (<italic>Sorghum bicolor</italic> L.) (Dura in Arabic) is a common source of human food an animal feed in most parts of the world including Africa, Central America, and South Asia<xref ref-type="bibr" rid="ridm1842690852">6</xref>. It is reported that 440,000 kilometers<xref ref-type="bibr" rid="ridm1842838044">2</xref> were devoted worldwide to <italic>Sorghum</italic> production in 2004 <xref ref-type="bibr" rid="ridm1842697620">7</xref>. Africa a lone produces about 20 mt of <italic>Sorghum</italic> per annum, which is equal to one-third of the world production. The major producing countries in Africa are,  Nigeria, Sudan, Ethiopia, Burkina Faso, Mali, Cameroon, Egypt, Niger, Tanzania, Chad, Uganda, and Mozambique <xref ref-type="bibr" rid="ridm1842693444">8</xref>.</p>
      <p>However, one of the problems which facing Sorghum production is the salinity, particularly in the arid, and semi arid regions where evapotransiration increases. Several studies revealed that the presence of broad intra-specific genetic variation in Sorghum varieties for salt stress with respect to their             early biomass production <xref ref-type="bibr" rid="ridm1842680604">9</xref>.  He added that high concentration of salinity inhibits and reduce young seedling development, but this depends on the cultivars. </p>
      <p>Several studies showed that Nitrogen is the limiting factor for most crops, particularly Sorghum <xref ref-type="bibr" rid="ridm1842678012">10</xref>.  A Study by <xref ref-type="bibr" rid="ridm1842664244">11</xref> and <xref ref-type="bibr" rid="ridm1842668780">12</xref> showed that sorghum grown as forage crop showed a persistent to salinity. The first stages of growth are decisive for establishment of effective population stand. However, the critical information is not yet available on growth response of this crop to soil salinity stress. Moreover, production potential is an important aspect in agricultural practices, for it reveals the actual yield of particular environment as influenced by agricultural and cultural practices, including fertilization <xref ref-type="bibr" rid="ridm1842666188">13</xref>. However, more production can be attained through further improvement of cultural practices and management <xref ref-type="bibr" rid="ridm1842656060">14</xref>. A researcher <xref ref-type="bibr" rid="ridm1842653900">15</xref> reported that incorporation of Nitrogen with organic sources into soil may maximize the plant ability to combat the hazard effects caused by irrigation with saline water. </p>
      <p>Furthermore, it is reported that increasing soil salinity has different effects on all growth parameters at 14, 70 and 100 days after sowing as well as the yield and its components<xref ref-type="bibr" rid="ridm1842649580">16</xref>.</p>
    </sec>
    <sec id="idm1842778412" sec-type="methods">
      <title>Methodology</title>
      <p>This study was carried out on the farm of College of Agriculture, University of                                            Bahri- at Alkadaro-Sudan, during the season 2014/2015. After the determination of the area of Interest (AOI) by GPS, a Randomized Completely Block Design (RCBD) was selected. It was arranged in three (3) blocks, each with three (3) replications and (4) treatments. Then the land was prepared by a Tractor (75 HP) (disc plow, leveling and furrowing).</p>
      <p>Seeds of Sorghum, cultivar locally known as Wad Ahmed (Son of Ahmed) were sown on ridges 70X30 cm apart in hills; at depths of 4-6 cm on 21/June/2014. The area was immediately irrigated after sowing. Thinning was done two weeks after        germination. Frequent interval irrigation of 8-10 days was practiced depending on weather conditions.      </p>
      <p>Two weeding were carried out manually, the first was two weeks after germination, and the second was thirty days after germination. Different levels of Nitrogen fertilizer (0, 25, 50, 75 kg/fed.) were applied one week after germination. </p>
      <p>The data pertaining the number of leaves per plant, Plant height (cm) and number of tillers per plant were taken at interval of 14 days. The collected data were statistically analyzed. The said data were presented in tables and graphs </p>
    </sec>
    <sec id="idm1842779708" sec-type="results">
      <title>Results</title>
      <p>The results of data analysis of leaf number, plant height, and number of tillers are presented in <xref ref-type="fig" rid="idm1842643396">Figure 1</xref>, <xref ref-type="fig" rid="idm1842641740">Figure 2</xref> and <xref ref-type="fig" rid="idm1842639292">Figure 3</xref>.</p>
    </sec>
    <sec id="idm1842780068" sec-type="discussion">
      <title>Discussion</title>
      <p>It is well known that soil salinity has negative effect on plants growth and development through osmotic stress and ion imbalance. This in turn affects the uptake; therefore, the plant physiological processes will be negatively influenced. Consequently, poor yield is expected. </p>
      <p>Leaf is one of the important agronomical traits which significantly contribute to grain yield. In this study, an application of different rates of Nitrogen fertilizer increased the leaf number throughout the growing period (14, 28, 42, 56 and 70 DAG). The Nitrogen rate of 75KgN/fed. produced greater leaf number (23, 26, 27, 29 and 32) at 14, 28, 42, 56 and 70 DAG respectively, compared to Nitrogen rates 50 (24, 25, 26, 28, 30), 25 (24, 25, 26, 28, 29) and 0 KgN/fed (24, 26, 27, 27, 27) at 14, 28, 42, 56 and 70 DAG respectively (<xref ref-type="fig" rid="idm1842643396">Figure 1</xref>). This result indicates that the leaf number increases with increase of Nitrogen levels. However, despite the crop response to fertilizer, but its growth is unhealthy. The crop appears pale green, reflecting the effect of soil type (saline) on plant growth (<xref ref-type="fig" rid="idm1842639436">Plate 1</xref>). Nevertheless, it is reported that the reduced concentrations of Ca 2+, K+, and Mg2+ in leaves under salinity could cause cation deficiency which reduces plant growth <xref ref-type="bibr" rid="ridm1842645188">17</xref><sup>.</sup></p>
      <p>As far as the plant height concern, the results reflected that all treatments positively increased the plant height including the control (Figure 2). All treatments including the control produced similar plant height of 58- 59 cm, which is shorter than plants grown in non saline soil. An experiment conducted in Faisalabad, Pakistan to investigate the effect of four levels of Nitrogen (0, 50, 75 and 100 kg N/acre) on agronomic attributes of Sorghum, revealed the maximum plant height was obtained by application of N4 (100 kg N/acre), produced plant height of 193.92, 195.24 and 192.79 cm in the first, second and the third cuttings respectively, followed by the treatment N3 (75 kg N/acre), produced plant height of 179.70 cm in first cutting, while the second and third cuttings revealed equal plant height (168.62 cm.)<xref ref-type="bibr" rid="ridm1842658148">18</xref> Furthermore, it is reported that the application of 60 to 240 kg (ha.sup.-1) of Nitrogen fertilizer had increased the plant heights to 269.6 and 320.32 cm, while, the levels of 180 and 240 kg (ha.sup.-1) showed non- significant differences <xref ref-type="bibr" rid="ridm1842627740">19</xref>.</p>
      <p>The result revealed no tillers in all treatments at 14 DAG. At 28 DAG, the control produced more tillers (2) compared with the other treatments (1). At 42 and 56 and 70 DAG, all treatments produced equal number of tillers (2). Generally, there is non- significant difference between the different treatments as far as the number of tillers concern. </p>
      <fig id="idm1842643396">
        <label>Figure 1.</label>
        <caption>
          <title> Effect of different Nitrogen levels on the average leaf number of Sorghum -Alkadaro-Sudan (2014).</title>
        </caption>
        <graphic xlink:href="images/image1.jpg" mime-subtype="jpg"/>
      </fig>
      <fig id="idm1842641740">
        <label>Figure 2.</label>
        <caption>
          <title> Effect of different Nitrogen levels on the average plant height (cm) of Sorghum at Alkadaro-Sudan (2014).</title>
        </caption>
        <graphic xlink:href="images/image2.jpg" mime-subtype="jpg"/>
      </fig>
      <fig id="idm1842639292">
        <label>Figure 3.</label>
        <caption>
          <title> Effect of different Nitrogen levels on number of Tillers of Sorghum–Alkadaro-Sudan (2014).</title>
        </caption>
        <graphic xlink:href="images/image3.jpg" mime-subtype="jpg"/>
      </fig>
      <fig id="idm1842639436">
        <label>Plate 1.</label>
        <caption>
          <title> Sorghun (Wad Ahmed), 42 DAG- grown is saline soil at AlKadaro- Sudan (2014)</title>
        </caption>
        <graphic xlink:href="images/image4.jpg" mime-subtype="jpg"/>
      </fig>
    </sec>
    <sec id="idm1842762012" sec-type="conclusions">
      <title>Conclusion and Recommendations</title>
      <sec id="idm1842762444">
        <title>Conclusion:</title>
        <p>The application of Nitrogen fertilizer to cereal crops in dry-land is vital. It depends on many factors, among these, the soil type, crop genotype, soil pH, environment, etc. In this study, responses of Sorghum (Wad Ahmed) to different levels of Nitrogen fertilizer showed variable results. The application of 75KgN/fed. revealed positive effect on all studied parameters, followed by 50 KgN/fed. and the control.  Although, the application of 75KgN/fed. showed an incremental effects on the studied traits, but the obtained measurements for all characters are beyond the expectation. This can be attributed to soil salinity. </p>
      </sec>
      <sec id="idm1842761796">
        <title> Recommendations</title>
        <p>Based on the results of this study, the saline soil (pH 7.5-7.8) of Alkadaro area is not suitable for growing Sorghum, particularly the cultivar Wad Ahmed.</p>
        <p>For biomass production, 75kg.N/fed. is recommended</p>
        <p>Further study is recommended to evaluate the response and performance of different Sorghum cultivars to different types and levels of fertlizers. </p>
      </sec>
    </sec>
  </body>
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