<?xml version="1.0" encoding="utf8"?>
 <!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.0 20120330//EN" "http://jats.nlm.nih.gov/publishing/1.0/JATS-journalpublishing1.dtd"> <article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" article-type="review-article" dtd-version="1.0" xml:lang="en">
  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">JAR</journal-id>
      <journal-title-group>
        <journal-title>Journal of Agronomy Research</journal-title>
      </journal-title-group>
      <issn pub-type="epub">2639-3166</issn>
      <publisher>
        <publisher-name>Open Access Pub</publisher-name>
        <publisher-loc>United States</publisher-loc>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.14302/issn.2639-3166.jar-22-4114</article-id>
      <article-id pub-id-type="publisher-id">JAR-22-4114</article-id>
      <article-categories>
        <subj-group>
          <subject>review-article</subject>
        </subj-group>
      </article-categories>
      <title-group>
        <article-title>Effect of Soil and Foliar Application of Plant Nutrients on Purple Blotch and Tip-Burn of Garlic </article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author">
          <name>
            <surname>Faruk</surname>
            <given-names>Md. Iqbal</given-names>
          </name>
          <xref ref-type="aff" rid="idm1850736244">1</xref>
          <xref ref-type="aff" rid="idm1850734228">*</xref>
        </contrib>
      </contrib-group>
      <aff id="idm1850736244">
        <label>1</label>
        <addr-line>Principal Scientific Officer, Plant Pathology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh. </addr-line>
      </aff>
      <aff id="idm1850734228">
        <label>*</label>
        <addr-line>Corresponding author</addr-line>
      </aff>
      <contrib-group>
        <contrib contrib-type="editor">
          <name>
            <surname>Abubaker</surname>
            <given-names>Haroun Mohamed Adam</given-names>
          </name>
          <xref ref-type="aff" rid="idm1850876124">1</xref>
        </contrib>
      </contrib-group>
      <aff id="idm1850876124">
        <label>1</label>
        <addr-line>Department of Crop Science (Agronomy), College of Agriculture, Bahri University- Alkadaru- Khartoum -Sudan.</addr-line>
      </aff>
      <author-notes>
        <corresp>
    
    Md. Iqbal Faruk, <addr-line>Principal Scientific Officer, Plant Pathology Division, Bangladesh Agricultural Research Institute, </addr-line><addr-line>Joydebpur</addr-line><addr-line>, Bangladesh</addr-line>. <email>mifaruk2012@yahoo.com</email></corresp>
        <fn fn-type="conflict" id="idm1844559156">
          <p>The authors have declared that no competing interests exist.</p>
        </fn>
      </author-notes>
      <pub-date pub-type="epub" iso-8601-date="2022-04-07">
        <day>07</day>
        <month>04</month>
        <year>2022</year>
      </pub-date>
      <volume>4</volume>
      <issue>3</issue>
      <fpage>39</fpage>
      <lpage>49</lpage>
      <history>
        <date date-type="received">
          <day>28</day>
          <month>02</month>
          <year>2022</year>
        </date>
        <date date-type="accepted">
          <day>14</day>
          <month>03</month>
          <year>2022</year>
        </date>
        <date date-type="online">
          <day>07</day>
          <month>04</month>
          <year>2022</year>
        </date>
      </history>
      <permissions>
        <copyright-statement>© </copyright-statement>
        <copyright-year>2022</copyright-year>
        <copyright-holder>Md. Iqbal Faruk</copyright-holder>
        <license xlink:href="http://creativecommons.org/licenses/by/4.0/" xlink:type="simple">
          <license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p>
        </license>
      </permissions>
      <self-uri xlink:href="http://openaccesspub.org/jar/article/1802">This article is available from http://openaccesspub.org/jar/article/1802</self-uri>
      <abstract>
        <p>An experiment was conducted in the field of Plant Pathology Division, BARI, Joydebpur to evaluate the efficacy of available plant nutrient for the development of purple blotch disease and                   tip-burn of onion. Eight different treatments viz. Potassium (MP fertilizer), Phosphorus (TSP                  fertilizer), Boron (Boron fertilizer), Zinc (ZnSO4 fertilizer), Calcium (CaSO<sub>4 </sub>fertilizer), Copper (CuSO<sub>4</sub>), Silicon (silica gel) and Manganese (MnSO<sub>4</sub> fertilizer) were tested against purple blotch disease and tip-burn of garlic. The present study revealed that soil and foliar application of plant nutrients viz. Potassium, Phosphorus, Boron, Zinc, Calcium, Copper, Silicon and Manganese gave appreciable reduction of purple blotch disease and tip-burn incidence and   increased plant growth parameters such as shoot and root growth as well as yield of garlic. Among the             nutrients Potassium, Phosphorus, Silicon, Zinc and Calcium were performed better for reducing purple blotch disease severity, tip-burn disease incidence and increasing plant growth as well as yield of garlic. Application of Boron and Manganese also performed better than control. So, soil and foliar application plant nutrients Potassium, Phosphorus, Silicon, Zinc, Boron and Manganese may be recommended for               tip-burn and purple blotch disease management and for garlic production in Bangladesh.</p>
      </abstract>
      <kwd-group>
        <kwd>Garlic</kwd>
        <kwd>purple blotch</kwd>
        <kwd>Alternaria porri</kwd>
        <kwd>tip burn</kwd>
        <kwd>plant nutrients</kwd>
      </kwd-group>
      <counts>
        <fig-count count="0"/>
        <table-count count="5"/>
        <page-count count="10"/>
      </counts>
    </article-meta>
  </front>
  <body>
    <sec id="idm1850596764" sec-type="intro">
      <title>Introduction</title>
      <p>Garlic (<italic>Allium</italic><italic>sativum</italic> L.) is one of the most important aromatic herbaceous annual spices under the family Alliaceae <xref ref-type="bibr" rid="ridm1845330508">1</xref>. It is the second most widely used cultivated Allium after onion<xref ref-type="bibr" rid="ridm1845328060">2</xref>. Garlic has been recognized all over the world as a valuable spice for cooking different dishes. In Bangladesh garlic cultivated in 73595 ha land and produces 485447 metric ton with an average yield of 4.43 t ha-1 <xref ref-type="bibr" rid="ridm1845336204">3</xref>. The yield is quite low in the world context. This low yield may be due to the               cultivation of the low yielding local varieties, incidence of diseases and insects, lack of technical knowledge etc. Both soil borne and foliar diseases are one of the major                  constrains for low yield of garlic in Bangladesh. Purple blotch caused by <italic>Alternaria </italic><italic>porri</italic> is a common destructive disease of garlic which adversely effects on garlic                   cultivation and and causes severe yield loss <xref ref-type="bibr" rid="ridm1845401716">4</xref><xref ref-type="bibr" rid="ridm1845190548">5</xref><xref ref-type="bibr" rid="ridm1845190404">6</xref><xref ref-type="bibr" rid="ridm1845185436">7</xref>. Now a days tip-burn become one of the major problem for garlic production. At present all the garlic cultivars are                       susceptible to purple blotch disease and tip burn.  In             Bangladesh, only fungicidal management is the effective means to manage the purple blotch disease but none of information available about tip burn problem. The control of plant diseases using pesticides raises serious concerns about food safety, environmental quality and pesticide resistance, which have dictated the need for alternative pest management techniques <xref ref-type="bibr" rid="ridm1845179756">8</xref>. So, it is important to find alternative measures to control plant diseases which do not harm the environment and at the same time increase yield and improve product quality <xref ref-type="bibr" rid="ridm1845175940">9</xref><xref ref-type="bibr" rid="ridm1845167652">10</xref><xref ref-type="bibr" rid="ridm1845163836">11</xref>. Nutrients are important for growth and development of plants and also microorganisms. They are important factors for disease suppression <xref ref-type="bibr" rid="ridm1845150700">12</xref>. All the essential nutrients can affect            disease severity <xref ref-type="bibr" rid="ridm1845147676">13</xref>. It is important to manage nutrient availability through fertilizers or change the soil                environment to influence nutrient availability, and in that way to control plant disease in an integrated pest                 management system <xref ref-type="bibr" rid="ridm1845147676">13</xref><xref ref-type="bibr" rid="ridm1845144652">14</xref>. In addition, nutrients can affect the development of a disease by affecting plant physiology or by affecting pathogens, or both of them. The level of nutrients can influence the plant growth, which can affect the microclimate, therefore affecting infection and sporulation of the pathogen <xref ref-type="bibr" rid="ridm1845142060">15</xref>. But unfortunately there is no available information about the effect of          nutrients on disease management in Bangladesh.                    Therefore the present study has taken to observe the                effect of plant nutrients on purple blotch disease and               tip-burn of garlic.</p>
    </sec>
    <sec id="idm1850602524" sec-type="materials">
      <title>Materials and Methods</title>
      <p>The experiment was conducted in the field of Plant Pathology Division, Bangladesh Agricultural                   Research Institute, Joydebpur, Gazipur during robi                   2015-16, 2016-17 and 2017-18 cropping seasons. There were 9 treatments viz. T<sub>1</sub>= Spray in the furrow soil during transplanting + Foliar spray of Potassium (MP fertilizer @2% water solution), T<sub>2</sub>= Spray in the furrow soil during transplanting + Foliar spray of Phosphorus (TSP fertilizer @2% water solution), T<sub>3</sub>= Spray in the furrow soil during transplanting + Foliar spray of Zinc (ZnSO<sub>4</sub> fertilizer @1% water solution), T<sub>4</sub>= Spray in the furrow soil during                transplanting + Foliar spray of Silicon (Silica gel @2%     water solution), T<sub>5</sub>= Spray in the furrow soil during               transplanting + Foliar spray of Boron (Boro fertilizer @1% water solution) T<sub>6</sub>= Spray in the furrow soil during                transplanting + Foliar spray of Calcium (CaSO<sub>4</sub> fertilizer @1% water solution), T<sub>7</sub>= Spray in the furrow soil during transplanting + Foliar spray of Manganese (MnSO<sub>4</sub>              fertilizer @ 1% water solution), T<sub>8</sub>= Spray in the furrow soil during transplanting + Foliar spray of Copper (CuSO<sub>4 </sub>@1% water solution) and T<sub>9</sub>= Control (only used                   recommended dose of fertilizers).  The unit plot size was 2 m x 2.5m. RCB design was followed with 3 replications. The variety BARI Rashun-1 was used.  The treatments were applied four times viz. 1<sup>st</sup> application at the time of seedling transplanting, 2<sup>nd</sup> application 40-45 days after seedling transplanting, 3<sup>rd</sup> application 15 days after 2<sup>nd</sup> application and 4<sup>th</sup> application 15 days after 3<sup>rd</sup>                 application.  Standard cultivation recommended by BARI was followed to grow garlic <xref ref-type="bibr" rid="ridm1845155236">16</xref>. During crop season                       necessary weeding, irrigation and other intercultural             operations were done as per recommendation of the crop.</p>
    </sec>
    <sec id="idm1850573980">
      <title>Data Collection</title>
      <p>Data were recorded on purple blotch disease              severity, tip-burn incidence, plant growth parameter such as plant height, shoot weight, root length and root weight and yield per unit area. Data on plant growth parameters were recorded 65 days after seedling transplanting.           Disease data were recorded 15 days after 4<sup>th</sup> time               treatments application. Data were calculated in terms of disease incidence and disease severity (PDI) by following formulae:</p>
      <fig id="idm1844641644">
        <graphic xlink:href="images/image1.jpg" mime-subtype="jpg"/>
      </fig>
      <fig id="idm1844617012">
        <graphic xlink:href="images/image2.jpg" mime-subtype="jpg"/>
      </fig>
      <p>The 0-5 disease scoring scale was used to estimate the disease severity (PDI-Percent Disease Index) of purple blotch complex of onion for each unit plot under each treatment. The scale was followed by Islam <italic>et al. </italic><xref ref-type="bibr" rid="ridm1845126348">17</xref> and Rahman and Rashid <xref ref-type="bibr" rid="ridm1845122388">18</xref> as described below:</p>
      <p>0 = no disease symptoms in the plant</p>
      <p>1 = a few spots towards the tip, covering less than 10% leaf area</p>
      <p>2 = several dark purplish brown patches covering less than 20% leaf area</p>
      <p>3 = several patches with paler outer zone, covering up to 40% leaf area</p>
      <p>4 = long streaks covering upto 75% leaf area or breaking of leaves / stalks from the centre</p>
      <p>5 = complete drying of the leaves/ stalks or breaking of the leaves / stalks from the base</p>
      <p>The percent data were converted into arcsine                          transformation values before statistical analysis. Data were analyzed statistically by using the MSTATC program. The treatment effects were compared by applying the least significant different (LSD) test at P=0.05 level.</p>
    </sec>
    <sec id="idm1850569876" sec-type="results">
      <title>
Results and Discussion</title>
      <sec id="idm1850571892">
        <title>Plant Growth</title>
        <p>Average plant height of onion under control was 27.27 cm plant<sup>-1</sup> in the first year, 44.67 cm plant<sup>-1</sup> in the second year and 39.33 cm plant<sup>-1</sup> in the third year             (<xref ref-type="table" rid="idm1844603716">Table 1</xref>). The plant height was increased to 31.73-38.80 cm plant<sup>-1</sup> in the first year, 47.47-57.00 cm plant<sup>-1</sup> in the second year and 42.00-54.00 cm plant<sup>-1</sup> in the third year due to soil and foliar application of different plant                   nutrients. In the first year, soli and foliar application TSP fertilizer, MP fertilizer and Silica gel gave higher plant height followed by ZnSO<sub>4</sub> fertilizer and MnSO<sub>4 </sub>fertilizer and (<xref ref-type="table" rid="idm1844603716">Table 1</xref>). Lower increased of plant height over              control was recorded from the treatment CuSO<sub>4</sub> followed by CaSO<sub>4</sub> fertilizer and Boron fertilizer. In the second year, the highest plant height was recored from MP fertilizer treatment followed by TSP fertilizer, Silica gel, ZnSO<sub>4</sub>               fertilizer, MnSO<sub>4 </sub>fertilizer and CaSO<sub>4</sub> fertilizer (<xref ref-type="table" rid="idm1844603716">Table 1</xref>). The least effective treatment in increasing of plant over control was recorded from CuSO<sub>4 </sub>followed by Boron             fertilizer treatment.  In the third year, the all the treatment gave significantly higher plant height compared to control except CuSO<sub>4</sub> where the plant height was lower than other treatment (<xref ref-type="table" rid="idm1844603716">Table 1</xref>).</p>
        <table-wrap id="idm1844603716">
          <label>Table 1.</label>
          <caption>
            <title> Effect of soil treatment and foliar application different nutrients on the plant growth of garlic during three consecutive years </title>
          </caption>
          <table rules="all" frame="box">
            <tbody>
              <tr>
                <td>Soil and foliar application of          different plant nutrient with dose</td>
                <td colspan="3">Plant height (cm)</td>
                <td colspan="3">Plant weight (gplant<sup>-1</sup>)</td>
              </tr>
              <tr>
                <td/>
                <td>2016-17</td>
                <td>2017-18</td>
                <td>2018-19</td>
                <td>2016-17</td>
                <td>2017-18</td>
                <td>2018-19</td>
              </tr>
              <tr>
                <td>Potassium (MP fertilizer @2%)</td>
                <td>36.40 a</td>
                <td>57.00 a</td>
                <td>54.00 a</td>
                <td>15.37 a</td>
                <td>22.53 a</td>
                <td>23.17 a</td>
              </tr>
              <tr>
                <td>Phosphorus (TSP fertilizer @2%)</td>
                <td>38.80 a</td>
                <td>53.93 ab</td>
                <td>54.00 a</td>
                <td>15.80 a</td>
                <td>21.87 ab</td>
                <td>22.00 a</td>
              </tr>
              <tr>
                <td>Zinc (ZnSO<sub>4</sub> fertilizer @1%)</td>
                <td>33.60 b</td>
                <td>53.20 b</td>
                <td>53.00 a</td>
                <td>13.87 b</td>
                <td>21.47 ab</td>
                <td>21.90 a</td>
              </tr>
              <tr>
                <td>Silicon (Silica gel @2%)</td>
                <td>38.67 a</td>
                <td>53.43 b</td>
                <td>51.67 a</td>
                <td>15.27 a</td>
                <td>20.73 ab</td>
                <td>21.30 a</td>
              </tr>
              <tr>
                <td>Boron (Boro fertilizer @1%)</td>
                <td>31.87 bc</td>
                <td>49.07 cd</td>
                <td>51.33 a</td>
                <td>12.53 c</td>
                <td>19.53 bc</td>
                <td>20.37 ab</td>
              </tr>
              <tr>
                <td>Calcium (CaSO<sub>4</sub> fertilizer @1%)</td>
                <td>31.73 bc</td>
                <td>51.40 bc</td>
                <td>49.67 a</td>
                <td>12.20 c</td>
                <td>21.40 ab</td>
                <td>19.93 ab</td>
              </tr>
              <tr>
                <td>Manganese (MnSO<sub>4 </sub>fertilizer @1%)</td>
                <td>33.47 b</td>
                <td>53.13 b</td>
                <td>51.33 a</td>
                <td>12.80 c</td>
                <td>19.87 ab</td>
                <td>20.37 ab</td>
              </tr>
              <tr>
                <td>Copper (CuSO<sub>4</sub> @1%)</td>
                <td>29.40 cd</td>
                <td>47.47 de</td>
                <td>42.00 b</td>
                <td>10.27 d</td>
                <td>16.93 cd</td>
                <td>17.10 bc</td>
              </tr>
              <tr>
                <td>Control</td>
                <td>27.27 d</td>
                <td>44.67 e</td>
                <td>39.33 b</td>
                <td>8.20 e</td>
                <td>15.93 d</td>
                <td>14.20 c</td>
              </tr>
              <tr>
                <td>LSD (P=0.05)</td>
                <td>2.507</td>
                <td>3.523</td>
                <td>4.987</td>
                <td>0.7683</td>
                <td>2.79</td>
                <td>3.963</td>
              </tr>
            </tbody>
          </table>
        </table-wrap>
        <p>In first year, the plant weight of garlic was 8.20 g plant<sup>-1</sup> under control. It increased to 10.27-15.80 g plant<sup>-1</sup> due to soil and foliar application of different nutrients (<xref ref-type="table" rid="idm1844603716">Table 1</xref>). Soil and foliar application of TSP fertilizer, MP fertilizer and Silica gel gave higher plant height followed ZnSO<sub>4 </sub>fertilizer treatment. The least effective treatment to increase plant weight was CuSO<sub>4</sub> followed by and CaSO<sub>4</sub> fertilizer<sub>,</sub> Boron fertilizer and MnSO<sub>4 </sub>treatments. In second year, the lowest plant weight of onion was 16.93 g plant<sup>-1</sup> recorded in the control. Soil and foliar application of MP fertilizer gave the highest plant weight 22.53 g plant<sup>-1           </sup> followed by the TSP fertilizer,ZnSO<sub>4</sub> fertilizer, CaSO<sub>4                 </sub>fertilizer, Silica gel and MnSO<sub>4 </sub>treatments where the plant weight was 21.87, 21.47, 21.40, 20.73 and 19.87 g plant<sup>-1</sup>, respectively (<xref ref-type="table" rid="idm1844603716">Table 1</xref>). In third year, the lowest plant weight of onion was 14.20 g plant<sup>-1</sup> recorded in the                control. Soil and foliar application of MP fertilizer, TSP fertilizer, Silica gel and ZnSO<sub>4 </sub>fertilizer gave higher plant height followed by Boro fertilizer, CaSO<sub>4</sub> and MnSO<sub>4                     </sub>treatments (<xref ref-type="table" rid="idm1844603716">Table 1</xref>). In all the years the least effective treatment was CuSO<sub>4</sub> treatment (<xref ref-type="table" rid="idm1844603716">Table 1</xref>). </p>
      </sec>
      <sec id="idm1850519212">
        <title>Root Growth</title>
        <p>Soil and foliar application of different plant          nutrients showed positive effects on root growth of garlic as compared to control (<xref ref-type="table" rid="idm1844493572">Table 2</xref>). In first year, higher root length 8.67 cm and 8.60 was recorded from Silica gel and MP fertilizer treatments followed by MnSO<sub>4 </sub>fertilizer, TSP fertilizer, Boron fertilizer, CaSO<sub>4</sub> fertilizer and ZnSO<sub>4</sub> where the root length was 7.80, 7.73, 7.47, 7.27 and 7.20 cm, respectively and the minimum root length 6.10 cm was recorded from control (<xref ref-type="table" rid="idm1844493572">Table 2</xref>). In the second and third years, all the treatments gave significantly higher root length over control except CuSO<sub>4</sub> wher root length was significantly lower than other treatments (<xref ref-type="table" rid="idm1844493572">Table 2</xref>). In all years the least effective treatment was CuSO<sub>4 </sub>followed by CaSO<sub>4</sub> (<xref ref-type="table" rid="idm1844493572">Table 2</xref>). Root weight under control was 0.53, 1.20 and 1.57 g/plant in the first year, second year and third year, respectively. The root weight was increase to 0.73-1.33, 1.53-1.87 and 1.83-2.93 g/plant in the first year, second year and third year, respectively due                 application of different treatments (<xref ref-type="table" rid="idm1844493572">Table 2</xref>). </p>
        <table-wrap id="idm1844493572">
          <label>Table 2.</label>
          <caption>
            <title> Effect of soil treatment and foliar application different nutrients on the root growth of garlic during three consecutive years </title>
          </caption>
          <table rules="all" frame="box">
            <tbody>
              <tr>
                <td>Soil and foliar application of         different plant nutrient with dose</td>
                <td colspan="3">Root length (cm)</td>
                <td colspan="3">Root weight (gplant<sup>-1</sup>)</td>
              </tr>
              <tr>
                <td/>
                <td>2016-17</td>
                <td>2017-18</td>
                <td>2018-19</td>
                <td>2016-17</td>
                <td>2017-18</td>
                <td>2018-19</td>
              </tr>
              <tr>
                <td>Potassium (MP fertilizer @2%)</td>
                <td>8.60 a</td>
                <td>6.73 a</td>
                <td>8.00 a</td>
                <td>1.00 ab</td>
                <td>1.73ab</td>
                <td>2.60 ab</td>
              </tr>
              <tr>
                <td>Phosphorus (TSP fertilizer @2%)</td>
                <td>7.73 ab</td>
                <td>7.07 a</td>
                <td>7.73 a</td>
                <td>1.07 a</td>
                <td>1.87a</td>
                <td>2.93a</td>
              </tr>
              <tr>
                <td>Zinc (ZnSO<sub>4</sub> fertilizer @1%)</td>
                <td>7.20 bc</td>
                <td>6.60 a</td>
                <td>7.73 a</td>
                <td>0.80 bc</td>
                <td>1.80 ab</td>
                <td>2.67 ab</td>
              </tr>
              <tr>
                <td>Silicon (Silica gel @2%)</td>
                <td>8.67 a</td>
                <td>6.47 a</td>
                <td>7.27 a</td>
                <td>1.33 a</td>
                <td>1.87 a</td>
                <td>2.77 ab</td>
              </tr>
              <tr>
                <td>Boron (Boron fertilizer @1%)</td>
                <td>7.47 bc</td>
                <td>6.73 a</td>
                <td>7.17 a</td>
                <td>0.73 cd</td>
                <td>1.80 ab</td>
                <td>2.47 ab</td>
              </tr>
              <tr>
                <td>Calcium (CaSO<sub>4</sub> fertilizer @1%)</td>
                <td>7.27 bc</td>
                <td>6.73 a</td>
                <td>7.47 a</td>
                <td>0.73 cd</td>
                <td>1.67 ab</td>
                <td>2.73 ab</td>
              </tr>
              <tr>
                <td>Manganese (MnSO<sub>4 </sub>fertilizer @1%)</td>
                <td>7. 80 ab</td>
                <td>6.60 a</td>
                <td>7.47 a</td>
                <td>0.93 abc</td>
                <td>1.67 ab</td>
                <td>2.33 bc</td>
              </tr>
              <tr>
                <td>Copper (CuSO<sub>4</sub> @1%)</td>
                <td>6.73 cd</td>
                <td>5.20 b</td>
                <td>5.83 b</td>
                <td>0.77 c</td>
                <td>1.53 b</td>
                <td>1.83 cd</td>
              </tr>
              <tr>
                <td>Control</td>
                <td>6.10 d</td>
                <td>4.67 b</td>
                <td>5.23 b</td>
                <td>0.53 d</td>
                <td>1.20 c</td>
                <td>1.57 d</td>
              </tr>
              <tr>
                <td>LSD (P=0.05)</td>
                <td>0.968</td>
                <td>0.654</td>
                <td>1.099</td>
                <td>0.232</td>
                <td>0.268</td>
                <td>0.581</td>
              </tr>
            </tbody>
          </table>
        </table-wrap>
      </sec>
      <sec id="idm1850451508">
        <title>Tip Burn Disease Incidence</title>
        <p>In all the years, the incidence of tip burn of garlic was reduced significantly over control due to soil and   foliar application with different plant nutrients compared to control (<xref ref-type="table" rid="idm1844402156">Table 3</xref>). In the first year, application of MP      fertilizer gave the lowest tip-burn incidence 33.33%            followed by Silica gel, TSP fertilizer, MnSO<sub>4</sub> fertilizer, ZnSO<sub>4 </sub>fertilizer and Boron fertilizer treatments where the tip-burn incidence was 35.00%, 40.00%, 43.33%, 46.67%, and 46.67%, respectively (<xref ref-type="table" rid="idm1844402156">Table 3</xref>). Application of CuSO<sub>4</sub> gave higher 53.33% tip burn incidence followed by CaSO<sub>4</sub> with 51.67% tip burn incidence, respectively. The highest tip-burn incidence 71.67% was recorded in control               treatment. Application of MP fertilizer reduced 53.50%    tip-burn incidence followed by the application of Silica gel, TSP fertilizer, MnSO<sub>4</sub> fertilizer, ZnSO<sub>4 </sub>fertilizer and Boron fertilizer treatments where the reduction was 51.16%, 44.19%, 39.53%, 34.88% and 34.88%, respectively                 compared to control.  In the second year, all the                      treatments significantly reduced tip-burn incidence               compared to control except CuSO<sub>4</sub> where the tip burn             incidence was 48.33% which was significantly higher than other treatments (<xref ref-type="table" rid="idm1844402156">Table 3</xref>). The highest tip burn incidence 58.33% was recoded in control. Application of TSP                fertilizer reduced 60.00% tip-burn incidence followed by MP fertilizer, Silica gel, CaSO<sub>4 </sub>fertilizer, ZnSO<sub>4</sub>, MnSO<sub>4 </sub>and Boron fertilizer where the reduction of tip-burn incidence of garlic was 57.15%, 57.15%, 57.15%, 54.28%, 51.43% and 51.43%, respectively compared to control. More or less similar trend was also observed in the third year. The highest tip burn incidence 74.33% was reorded from                control treatment. Tip burn incidence drastically reduced to 39.67%-53.33% due to application different treatments. Application of MP fertilizer reduced 46.63% tip-burn              incidence followed by and TSP fertilizer, Silica gel, MnSO<sub>4</sub>, ZnSO<sub>4</sub>, Boron fertilizer and CaSO<sub>4 </sub>fertilizer where the             reduction of tip-burn incidence of garlic was 45.74%, 45.28%, 45.28%, 44.40%, 38.56% and 35.42%,                    respectively compared to control (<xref ref-type="table" rid="idm1844402156">Table 3</xref>).</p>
        <table-wrap id="idm1844402156">
          <label>Table 3.</label>
          <caption>
            <title> Effect of soil treatment and foliar application different nutrients on the incidence of tip burn of garlic during three consecutive years  </title>
          </caption>
          <table rules="all" frame="box">
            <tbody>
              <tr>
                <td>Soil and foliar application of different plant nutrient with dose</td>
                <td colspan="3">Tip burn incidence of garlic</td>
                <td colspan="3">Reduction of tip-burn incidence (%)</td>
              </tr>
              <tr>
                <td/>
                <td>2016-17</td>
                <td>2017-18</td>
                <td>2018-19</td>
                <td>2016-17</td>
                <td>2017-18</td>
                <td>2018-19</td>
              </tr>
              <tr>
                <td>Potassium (MP fertilizer @2%)</td>
                <td>33.33 d(35.22)</td>
                <td>25.00 c (29.92)</td>
                <td>39.67 c(38.99)</td>
                <td>53.50</td>
                <td>57.15</td>
                <td>46.63</td>
              </tr>
              <tr>
                <td>Phosphorus (TSP fertilizer @2%)</td>
                <td>40.00 cd(39.15)</td>
                <td>23.33 c (28.85)</td>
                <td>40.33 c(39.41)</td>
                <td>44.19</td>
                <td>60.00</td>
                <td>45.74</td>
              </tr>
              <tr>
                <td>Zinc (ZnSO<sub>4</sub> fertilizer @1%)</td>
                <td>46.67 bc(43.09)</td>
                <td>26.67 c (31.07)</td>
                <td>41.33 c(40.00)</td>
                <td>34.88</td>
                <td>54.28</td>
                <td>44.40</td>
              </tr>
              <tr>
                <td>Silicon (Silica gel @2%)</td>
                <td>35.00 d(36.18)</td>
                <td>25.00 c (29.80)</td>
                <td>40.67 c(39.62)</td>
                <td>51.16</td>
                <td>57.15</td>
                <td>45.28</td>
              </tr>
              <tr>
                <td>Boron (Boro fertilizer @1%)</td>
                <td>46.67 bc(43.08)</td>
                <td>28.33 c (32.14)</td>
                <td>45.67 c(39.50)</td>
                <td>34.88</td>
                <td>51.43</td>
                <td>38.56</td>
              </tr>
              <tr>
                <td>Calcium (CaSO<sub>4</sub> fertilizer @1%)</td>
                <td>51.67 b(45.96)</td>
                <td>25.00 c (29.92)</td>
                <td>48.00 bc(43.85)</td>
                <td>27.91</td>
                <td>57.15</td>
                <td>35.42</td>
              </tr>
              <tr>
                <td>Manganese (MnSO<sub>4 </sub>fertilizer @1%)</td>
                <td>43.33 bcd(41.07)</td>
                <td>28.33 c (32.14)</td>
                <td>40.67 c(39.61)</td>
                <td>39.54</td>
                <td>51.43</td>
                <td>45.28</td>
              </tr>
              <tr>
                <td>Copper (CuSO<sub>4</sub> @1%)</td>
                <td>53.33 b(46.91)</td>
                <td>48.33 b (43.08)</td>
                <td>53.33 b(46.92)</td>
                <td>25.58</td>
                <td>17.14</td>
                <td>28.25</td>
              </tr>
              <tr>
                <td>Control</td>
                <td>71.67 a(57.98)</td>
                <td>58.33 a (49.83)</td>
                <td>74.33 a(59.53)</td>
                <td>-</td>
                <td>-</td>
                <td>-</td>
              </tr>
              <tr>
                <td>LSD (P=0.05)</td>
                <td>6.788</td>
                <td>6.788</td>
                <td>6.918</td>
                <td>-</td>
                <td>-</td>
                <td>-</td>
              </tr>
            </tbody>
          </table>
        </table-wrap>
      </sec>
      <sec id="idm1850393524">
        <title>Purple Blotch Disease Severity</title>
        <p>All the treatments significantly reduced purple blotch disease severity compared to control during three consecutive years. In the first year all the treatments           significantly reduced the purple blotch disease severity than control (<xref ref-type="table" rid="idm1844299244">Table 4</xref>).  Soil and foliar application of              Application of Silica gel  reduced 59.66% purple blotch disease severity compared to control followed by TSP    fertilizer, MP fertilizer,  ZnSO<sub>4</sub> fertilizer, MnSO<sub>4 </sub>fertilizer, Boron fertilizer, CaSO<sub>4</sub> fertilizer and CuSO<sub>4</sub>treatments where the reduction of disease severity was 59.09%, 57.95%, 57.39%, 53.98%, 53.98% and 52.84%,                        respectively compared to control (<xref ref-type="table" rid="idm1844299244">Table 4</xref>). The highest purple blotch disease severity 58.67% was observed in control treatment. More or less similer trend of reduction of purple disease severity was observed in the second and third year’s trials. In the second year, all the treatments significantly reduced purple blotch disease severity               compared to control except CuSO<sub>4 </sub>where the purple blotch disease severity wassignificantly higher than other              treatments. The highest purple blotch disease severity 57.33% was observed in control treatment. Application of MP fertilizer and TSP fertilizer reduced 58.14% purple blotch disease severity compared to control followed by the application of ZnSO<sub>4, </sub>Silica gel,  Boron fertilizer, MnSO<sub>4 </sub>and CaSO<sub>4 </sub>treatments where the reduction was 55.82%, 54.65%, 53.48%, 53.48% and 52.33%, respectively than control. In the third year, application of MP fertilizer         reduced 50.00% disease severity followed by the              application of TSP fertilizer, Silica gel, ZnSO<sub>4</sub>,CaSO<sub>4</sub>, MnSO<sub>4 </sub>and Boron fertilizer treatments where the                  reduction was 48.78%, 48.21%, 47.63%, 47.02%, 45.84% and 44.64%, respectively compared to control. The               highest purple blotch disease severity 56.00% was           observed in control treatment. In all the years the least effective treatment in reduction of purple disease severity was CuSO<sub>4</sub> treatment.</p>
        <table-wrap id="idm1844299244">
          <label>Table 4.</label>
          <caption>
            <title> Effect of soil treatment and foliar application different nutrients against purple blotch disease severity of garlic during three consecutive years </title>
          </caption>
          <table rules="all" frame="box">
            <tbody>
              <tr>
                <td>Soil and foliar application of              different plant nutrient with dose</td>
                <td colspan="3">Severity of purple blotch disease of garlic (PDI)</td>
                <td colspan="3">Reduction of severity of purple blotch disease (%)</td>
              </tr>
              <tr>
                <td/>
                <td>2016-17</td>
                <td>2017-18</td>
                <td>2018-19</td>
                <td>2016-17</td>
                <td>2017-18</td>
                <td>2018-19</td>
              </tr>
              <tr>
                <td>Potassium (MP fertilizer @2%)</td>
                <td>24.67 b(29.76)</td>
                <td>24.00 d (29.30)</td>
                <td>28.00 c (31.93)</td>
                <td>57.95</td>
                <td>58.14</td>
                <td>50.00</td>
              </tr>
              <tr>
                <td>Phosphorus (TSP fertilizer @2%)</td>
                <td>24.00 b(29.21)</td>
                <td>24.00 d (29.32)</td>
                <td>28.67 c (32.37)</td>
                <td>59.09</td>
                <td>58.14</td>
                <td>48.78</td>
              </tr>
              <tr>
                <td>Zinc (ZnSO<sub>4</sub> fertilizer @1%)</td>
                <td>25.00 b(29.94)</td>
                <td>25.33 cd (30.22)</td>
                <td>29.33 c (32.77)</td>
                <td>57.39</td>
                <td>55.82</td>
                <td>47.63</td>
              </tr>
              <tr>
                <td>Silicon (Silica gel @2%)</td>
                <td>23.67 b(29.02)</td>
                <td>26.00 cd (30.65)</td>
                <td>29.00 c (32.56)</td>
                <td>59.66</td>
                <td>54.65</td>
                <td>48.21</td>
              </tr>
              <tr>
                <td>Boron (Boro fertilizer @1%)</td>
                <td>27.00 b(31.31)</td>
                <td>26.67 cd (31.08)</td>
                <td>31.00bc (33.82)</td>
                <td>53.98</td>
                <td>53.48</td>
                <td>44.64</td>
              </tr>
              <tr>
                <td>Calcium (CaSO<sub>4</sub> fertilizer @1%)</td>
                <td>27.00 b(31.30)</td>
                <td>27.33 c (31.52)</td>
                <td>29.67 c (32.99)</td>
                <td>53.98</td>
                <td>52.33</td>
                <td>47.02</td>
              </tr>
              <tr>
                <td>Manganese (MnSO<sub>4 </sub>fertilizer @1%)</td>
                <td>25.00 b(29.96)</td>
                <td>26.67 cd (31.08)</td>
                <td>30.33 c (33.41)</td>
                <td>57.39</td>
                <td>53.48</td>
                <td>45.84</td>
              </tr>
              <tr>
                <td>Copper (CuSO<sub>4</sub> @1%)</td>
                <td>27.67 b(31.70)</td>
                <td>45.33 b (42.32)</td>
                <td>36.00 b (36.85)</td>
                <td>52.84</td>
                <td>20.93</td>
                <td>35.71</td>
              </tr>
              <tr>
                <td>Control</td>
                <td>58.67 a(50.05)</td>
                <td>57.33 a (49.22)</td>
                <td>56.00 a (48.46)</td>
                <td>-</td>
                <td>
                  <bold>-</bold>
                </td>
                <td>-</td>
              </tr>
              <tr>
                <td>LSD (P=0.05)</td>
                <td>5.386</td>
                <td>2.06</td>
                <td>3.051</td>
                <td>-</td>
                <td>-</td>
                <td>-</td>
              </tr>
            </tbody>
          </table>
        </table-wrap>
      </sec>
      <sec id="idm1850347420">
        <title>Crop Yield</title>
        <p>In all the years, soil and foliar application of             different plant nutrientsgave signicantly higher yield of garlic (<xref ref-type="table" rid="idm1844169116">Table 5</xref>). In first year, the lowest yield of 3.16 t/ha was found under control. The yield was increased to            4.20-6.42 t/ha due to application of different treatments. Soil and foliar application of Silica gel gave the highest yield 6.42 tha<sup>-1</sup> followed by the application of MP fertilizer, TSP fertilizer, ZnSO<sub>4 </sub>fertilizer, MnSO<sub>4 </sub>fertilizer and Boro fertilizer where the yield was 5.28, 5.28, 5.20, 5.19 and 4.75 tha<sup>-1</sup>, respectively. Application of CuSO<sub>4</sub> gave lower yield 4.18 tha<sup>-1</sup> followed by the application of CaSO<sub>4 </sub>where the yield was 4.20 tha<sup>-1</sup>. The maximum yield increased 50.78% compared to control was obtained by Silica gel followed by MP fertilizer, TSP fertilizer, ZnSO<sub>4 </sub>fertilizer, MnSO<sub>4 </sub>fertilizer and Boro fertilizer where the yield was 40.15%, 40.15%, 39.23%, 39.11% and 33.47%,  respectively higher than control (<xref ref-type="table" rid="idm1844299244">Table 4</xref>). The lowest               increase was achieved with CuSO<sub>4</sub> followed by CaSO<sub>4 </sub>where yield was 24.40% and 24.76%, respectively higher than control. In the 2<sup>nd</sup> year, average yield of garlic was 4.22 t/ha under control and 4.85 to 6.77 t/ha under               treated plots. All the treatments gave significantly higher yield of garlic than control exceptCuSO<sub>4 </sub>treatment where the yield was significantly lower than other treatments.  Application of MP fertilizer gave the 37.67% higher yield compared to control which was followed by the                    application TSP fertilizer, Silica gel, Boron fertilizer,  ZnSO<sub>4</sub> fertilizer,MnSO<sub>4</sub> fertilizerand CaSO<sub>4 </sub>fertilizer where the yield was 34.78%, 34.78%, 32.59%, 29.19%, 29.19% and 29.19%, respectively higher than control (<xref ref-type="table" rid="idm1844169116">Table 5</xref>). In the 3<sup>rd</sup> year, the highest yield was 7.15 tha<sup>-1 </sup>obtained with the application of MP fertilizer followed by the application of TSP fertilizer, Silica gel<sub>, </sub>Boron fertilizer, ZnSO<sub>4,</sub> MnSO4andCaSO<sub>4 </sub>where  the yield was 6.79, 6.69, 6.68, 6.61, 6.51 and 6.45 tha<sup>-1</sup>. The lowest yield of garlic 4.72 tha<sup>-1 </sup>was recorded in control treatment which was followed by CuSO<sub>4 </sub>with the yield of 5.35 tha<sup>-1</sup>.Application of MP fertilizer gave the 33.98% higher yield compared to control which was followed by the application TSP fertilizer, Silica gel<sub>, </sub>Boron fertilizer, ZnSO<sub>4,</sub> MnSO4andCaSO<sub>4 </sub>where the yield was 30.48%, 29.45%, 29.34%, 28.59%, 27.50% and 26.82%, respectively higher than control (<xref ref-type="table" rid="idm1844169116">Table 5</xref>). </p>
        <table-wrap id="idm1844169116">
          <label>Table 5.</label>
          <caption>
            <title> Effect of soil treatment and foliar application different nutrients on the yield of garlic during three consecutive years</title>
          </caption>
          <table rules="all" frame="box">
            <tbody>
              <tr>
                <td>Soil and foliar application of different plant nutrient with dose</td>
                <td colspan="3">Yield (tha<sup>-1</sup>)</td>
                <td colspan="3">Yield increased over control (%)</td>
              </tr>
              <tr>
                <td/>
                <td>2016-17</td>
                <td>2017-18</td>
                <td>2018-19</td>
                <td>2016-17</td>
                <td>2017-18</td>
                <td>2018-19</td>
              </tr>
              <tr>
                <td>Potassium(MP fertilizer @2%)</td>
                <td>5.28 b</td>
                <td>6.77 a</td>
                <td>7.15 a</td>
                <td>40.15</td>
                <td>37.67</td>
                <td>33.98</td>
              </tr>
              <tr>
                <td>Phosphorus(TSP fertilizer @2%)</td>
                <td>5.28 b</td>
                <td>6.47 a</td>
                <td>6.79 ab</td>
                <td>40.15</td>
                <td>34.78</td>
                <td>30.48</td>
              </tr>
              <tr>
                <td>Zinc(ZnSO<sub>4</sub> fertilizer @1%)</td>
                <td>5.20 bc</td>
                <td>5.96 a</td>
                <td>6.61 b</td>
                <td>39.23</td>
                <td>29.19</td>
                <td>28.59</td>
              </tr>
              <tr>
                <td>Silicon(Silica gel @2%)</td>
                <td>6.42 a</td>
                <td>6.47 a</td>
                <td>6.69 ab</td>
                <td>50.78</td>
                <td>34.78</td>
                <td>29.45</td>
              </tr>
              <tr>
                <td>Boron(Boro fertilizer @1%)</td>
                <td>4.75 bc</td>
                <td>6.26 a</td>
                <td>6.68 b</td>
                <td>33.47</td>
                <td>32.59</td>
                <td>29.34</td>
              </tr>
              <tr>
                <td>Calcium(CaSO<sub>4</sub> fertilizer @1%)</td>
                <td>4.20 c</td>
                <td>5.96 a</td>
                <td>6.45 b</td>
                <td>24.76</td>
                <td>29.19</td>
                <td>26.82</td>
              </tr>
              <tr>
                <td>Manganese(MnSO<sub>4 </sub>fertilizer @1%)</td>
                <td>5.19 bc</td>
                <td>5.96 a</td>
                <td>6.51 b</td>
                <td>39.11</td>
                <td>29.19</td>
                <td>27.50</td>
              </tr>
              <tr>
                <td>Copper(CuSO<sub>4</sub> @1%)</td>
                <td>4.18 cd</td>
                <td>4.85 b</td>
                <td>5.35 c</td>
                <td>24.40</td>
                <td>12.98</td>
                <td>11.78</td>
              </tr>
              <tr>
                <td>Control</td>
                <td>3.16 d</td>
                <td>4.22 b</td>
                <td>4.72 d</td>
                <td>-</td>
                <td>-</td>
                <td>-</td>
              </tr>
              <tr>
                <td>LSD (P=0.05)</td>
                <td>1.03</td>
                <td>1.03</td>
                <td>0.471</td>
                <td>-</td>
                <td>-</td>
                <td>-</td>
              </tr>
            </tbody>
          </table>
        </table-wrap>
        <p>From this study it is observed that application different plant nutrient have significant effect on plant growth, decreased purple blotch diseases severity and tip burn incidence as well as increase yield of garlic. Among the nutrients MP fertilizer, TSP fertilizer, ZnSO<sub>4, </sub>Silica gel, Boron fertilizer and CaSO<sub>4</sub> were performed better than other treatments for reducing purple blotch disease             severity, tip-burn and increasing plant growth as well as yield of garlic. Different workers reported that the use of fertilizers produces a more direct means of using                  nutrients to reduce the severity of many                                diseases <xref ref-type="bibr" rid="ridm1845142060">15</xref><xref ref-type="bibr" rid="ridm1845175940">9</xref><xref ref-type="bibr" rid="ridm1845117852">19</xref><xref ref-type="bibr" rid="ridm1845133188">20</xref><xref ref-type="bibr" rid="ridm1845126852">21</xref>. Dordas <xref ref-type="bibr" rid="ridm1845102028">22</xref> reported that potassium fertilization can reduce the intensity of several infectious diseases of obligate and facultative parasites. A number of studies have shown that application Potassium, Phosphorus, Boron, Zinc, Calcium, Silicon and Manganese can reduce fungal, bacterial and viral diseases of many crops <xref ref-type="bibr" rid="ridm1845147676">13</xref><xref ref-type="bibr" rid="ridm1845101452">23</xref><xref ref-type="bibr" rid="ridm1845098788">24</xref><xref ref-type="bibr" rid="ridm1845096772">25</xref><xref ref-type="bibr" rid="ridm1845107356">26</xref><xref ref-type="bibr" rid="ridm1845076580">27</xref><xref ref-type="bibr" rid="ridm1845073484">28</xref><xref ref-type="bibr" rid="ridm1845070244">29</xref><xref ref-type="bibr" rid="ridm1845064988">30</xref><xref ref-type="bibr" rid="ridm1845079388">31</xref><xref ref-type="bibr" rid="ridm1845043844">32</xref><xref ref-type="bibr" rid="ridm1845039812">33</xref><xref ref-type="bibr" rid="ridm1845038660">34</xref>. Agrios <xref ref-type="bibr" rid="ridm1845150700">12</xref> reported that plant           nutrients are important for growth and development of plants and also microorganisms and also important factors in disease control.</p>
      </sec>
    </sec>
    <sec id="idm1850281780" sec-type="conclusions">
      <title>Conclusion</title>
      <p>The present study revealed that soil and foliar application of plant nutrients viz. Potassium, Phosphorus, Boron, Zinc, Calcium, Copper, Silicon and Manganese gave appreciable reduction of purple blotch disease and            tip-burn incidence and increased plant growth parameters such as shoot and root growth as well as yield of garlic. Among the nutrients Potassium, Phosphorus, Silicon, Zinc and Calcium were performed better for reducing purple blotch disease severity, tip-burn disease incidence and increasing plant growth as well as yield of garlic. So, soil and foliar application plant nutrients Potassium                  Phosphorus, Silicon, Zinc, Boron and Manganese may be recommended for tip-burn and purple blotch disease   management and for garlic production in Bangladesh.</p>
    </sec>
  </body>
  <back>
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