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  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">JSCE</journal-id>
      <journal-title-group>
        <journal-title>Journal of Skin Cancer Epidemiology</journal-title>
      </journal-title-group>
      <issn pub-type="epub">0000-0000</issn>
      <issn pub-type="ppub">0000-0000</issn>
      <publisher>
        <publisher-name>Open Access Pub</publisher-name>
        <publisher-loc>United States</publisher-loc>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="publisher-id">JSCE-22-4073</article-id>
      <article-categories>
        <subj-group>
          <subject>research-article</subject>
        </subj-group>
      </article-categories>
      <title-group>
        <article-title>New Approach in Treatment of Cancer &amp; Viral Infection by Apis Mellifera L Venom Extracts (Honeybees Venom)</article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author">
          <name>
            <surname>Samia</surname>
            <given-names>Ahmed Kamal</given-names>
          </name>
          <xref ref-type="aff" rid="idm1850407012">1</xref>
          <xref ref-type="corresp" rid="cor1">*</xref>
        </contrib>
      </contrib-group>
      <aff id="idm1850407012">
        <label>1</label>
        <addr-line>Department of Virology, Agricultural Research Center, Cairo, Egypt</addr-line>
      </aff>
      <contrib-group>
        <contrib contrib-type="editor">
          <name>
            <surname>Fatma</surname>
            <given-names>Taher</given-names>
          </name>
          <xref ref-type="aff" rid="idm1850271324">1</xref>
        </contrib>
      </contrib-group>
      <aff id="idm1850271324">
        <label>1</label>
        <addr-line>Department of Electronic and             Computer Engineering, Khalifa University, Abu Dhabi</addr-line>
      </aff>
      <author-notes>
        <corresp id="cor1">Correspondence: Samia Ahmed Kamal, Department of Virology, Agricultural Research Center, Cairo, Egypt; Email: <email>selkabany@yahoo.com</email>.</corresp>
        <fn fn-type="conflict" id="idm1850460548">
          <p>The authors have declared that no competing interests exist.</p>
        </fn>
      </author-notes>
      <pub-date pub-type="epub" iso-8601-date="2022-02-06">
        <day>06</day>
        <month>02</month>
        <year>2022</year>
      </pub-date>
      <volume>1</volume>
      <issue>1</issue>
      <fpage>16</fpage>
      <lpage>20</lpage>
      <history>
        <date date-type="received">
          <day>21</day>
          <month>01</month>
          <year>2022</year>
        </date>
        <date date-type="accepted">
          <day>02</day>
          <month>02</month>
          <year>2022</year>
        </date>
        <date date-type="online">
          <day>06</day>
          <month>02</month>
          <year>2022</year>
        </date>
      </history>
      <permissions>
        <copyright-statement>© </copyright-statement>
        <copyright-year>2022</copyright-year>
        <copyright-holder>Samia Ahmed Kamal</copyright-holder>
        <license xlink:href="http://creativecommons.org/licenses/by/4.0/" xlink:type="simple">
          <license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p>
        </license>
      </permissions>
      <self-uri xlink:href="http://openaccesspub.org/jsce/article/1771">This article is available from http://openaccesspub.org/jsce/article/1771</self-uri>
      <abstract>
        <p>Cancer cells need strong drug to be              eliminated. Cancer lesions cure could achieve by topical application of crude bee venom. Bee venom medication used to prevent malignancies in groups most at risk (predisposing factors). Bee venom crosses the blood brain barriers because its               components are very small. However, Bee venom contraindicated administered by intravenous        injection because it’s hemolytic substance, mellitin which is powerful anticoagulant. However, the                cationic peptides mellitin govern the mode of action of bee venom as anticancer and antiviral in vivo; <xref ref-type="bibr" rid="ridm1849918820">1</xref> there is a negative charge on cancer cells, viral                 infected cells, diseased cells, and generally any cells that contain toxins or damage, and viruses are                carrying negative charge even when it is outside the living body. <xref ref-type="bibr" rid="ridm1849912700">2</xref>Bee venom component (melittin)              carries a positive charge, it destruct negatively charged cancer cells. <xref ref-type="bibr" rid="ridm1849925524">3</xref> The role that the herpes             virus is likely to play in increasing the severity of                 cancerous diseases, worsen the conditions: herpes viruses are opportunistic viruses that strike the body whose immunity is weakened for any reason.                  Therefore, the role of herpes virus must be                    neutralized when you planning to treat a cancer               patient. Fortunately, bee venom is a powerful               antiviral, and thus we hit three birds with one stone, that is, we kill cancer cells, kill opportunistic viruses, and improve tissue immunity to participate in the fight against cancer and get rid of toxic exudates more efficiently.</p>
      </abstract>
      <kwd-group>
        <kwd>Honeybees</kwd>
        <kwd>mellitin</kwd>
        <kwd>Bee venom</kwd>
        <kwd>anticancer therapy</kwd>
        <kwd>antivirals</kwd>
        <kwd>cationic peptides</kwd>
        <kwd>allergy management</kwd>
      </kwd-group>
      <counts>
        <fig-count count="4"/>
        <table-count count="0"/>
        <page-count count="0"/>
      </counts>
    </article-meta>
  </front>
  <body>
    <sec id="idm1850270172">
      <title>Background </title>
      <p>Cancer cells need strong drug to be                    eliminated. When these cells multiply, they do not adhere to the type of tissue originated from, but are able to attack blood vessels and various tissues and secrete toxins capable of weakening immunity and stopping the immune system from doing its role. Also, the substances resulting from the proliferation of cancer cannot be eliminated by the body due to the defect that occurred in the microcirculation.</p>
      <p>Skin cancer can be treated with safe local                 treatment by whole bee venom, especially that cancer               located near or in vital organs such as the eyes, nose, ear, face and head. The cancer of the organs located under the skin also can be dealt with in the same way. The only problem in this regard is the lack of experience or                  education for these new methods that require practical experience and expertise. It is not just an effective drug against cancer, but a sub-specialty that must be studied because it will change the course of dealing with patients and completely different from what is known.<xref ref-type="bibr" rid="ridm1849918820">1</xref></p>
      <p>Each patient is a unique case, and the doctor must know all its details, so he cannot generalize. For example, bee venom concentration varies from stage to stage and varies from site of injection to another, and the reaction of people to the drug depends on unique factors as well.</p>
      <p>Example: the patient's age, general health status, degree of cancer, disease size and spread, extent and              location, each factor must be taken into consideration. As for bee venom, the doctor is the only person who will deal with it. The doctor must verify the source of bee venom and that it is not adulterated. He must also make sure of its concentration and even prepare it himself. The doctor must know the safe doses for each person individually according to his general health condition, and he must realize the degree of sensitivity of each person to this drug.<xref ref-type="bibr" rid="ridm1849912700">2</xref></p>
      <p>The doctor who will use this drug must do some scientific laboratory experiments on the culture cells that were extracted from the tumors and on experimental              animals before starting to use it for treatment, i.e. he must be an expert in this drug. A doctor, who knows the                   capabilities of this drug, will be able to use it and be               creative in treating patients in all circumstances. If the surgeon is able to fully understand the secrets of this            medicine, he will be creative during the treatment of                 internal tumors and during his surgery as well. For                   example, bee venom can stop the transmission of tumors to neighboring tissues and prevent its spread (injecting bee venom during surgery). Bee venom can applied to                    prevent tumors in groups most at risk of potential cancer (predisposing factors). Like smokers, elderly, as well as brain and nervous system tumors. This drug can reach the brain and all parts of the body through the blood.                 Whatever the way the drug is administered, it reaches various organs of the body. But, for example, may not be injected into the vein at all. But if taken by adding it to food, it helps prevent clots. The secrets of this drug are very huge and its applications are many, all of which are important.<xref ref-type="bibr" rid="ridm1849925524">3</xref></p>
      <p>
        <italic>Honey Bees’ Venom (Honey Bees Stings Extract) Innate Mode </italic>
        <italic>Of</italic>
        <italic> Action</italic>
      </p>
      <p>1. For example: Breast cancer: you can inject the drug inside the tumor, through the skin, the cancer cells died immediately, but the normal cells will not           affected or die, that because this drug work selectively and functionally, it is not a mechanical destruction of tissues, </p>
      <p>2. Examples: lesions of Lyme disease: (bacterium              Borrelia burgdorferi), bee venom treats the lesions itself, curing the effects of bacteria on tissues, enhance immune system to deals with the microbes, moreover kills the bacterium by another techniques. All this functions are highly complicated process, </p>
      <p>3. Example: in vitro experiments; bee venom dissolves the cancer cell lines; however in vivo; it only kill              cancer cells and enhance the vitality of normal cells. Why? The answer is the negative charge on abnormal cells inside the living organism. The viruses infected cells, the cancer cells, the cells contain parasites or bacteria, all these abnormal cells has charge on it, so that the drug targeted it and functionally remove it away from the normal tissue. </p>
      <p>4. Moreover, bee venom components are very small in size, but have certain type of genetic materials, i.e. have a mind and a mission. </p>
    </sec>
    <sec id="idm1850275644" sec-type="materials">
      <title>Materials &amp; Methods</title>
      <p>Bee venom must be prepared immediately before use to avoid oxidation. Then packaged in tubes containing a single dose and kept at minus 20 ℃.</p>
      <sec id="idm1850272476">
        <title>Preparation of Bee Venom Solution for Injection</title>
        <p>1 g + 3 liters distilled water</p>
      </sec>
      <sec id="idm1850272908">
        <title>Filtration Process</title>
        <p>1. Dissolve the medicine in a small amount of water, such as 20 ml of distilled water</p>
        <p>2. Then we prepare a special filter for the syringe; the size of the pores in the filter is 0.2 microns.</p>
        <p>3. We withdraw the solution with a syringe and then carry out the filtration process.</p>
        <p>4. Then pour the filtrate into the rest of the water.</p>
      </sec>
      <sec id="idm1850271900">
        <title>Bee Venom Extraction from the Honeybee’s Stings</title>
        <p>1. Electric set designed to collects bees stings operated by beekeepers at their apiaries  </p>
        <p>2. Wait until the evaporation of liquid and volatile             substances off the product, then collect bee venom when a crystalline material appear that tends to be white or light yellow. Such extract would lose a large amount of the liquid components of the stinging bag, as a result of drying; allergic reactions are lesser             effects for bee venom than the bee’s stings as the             experts in this field say that volatiles components                    contain the largest percentage of allergens.</p>
        <p>3. This dry extract is the crude bee venom (whole bee venom).</p>
      </sec>
      <sec id="idm1850254788">
        <title>Bee Venom Allergy Examination</title>
        <p>Some people are allergic to bee stings, and some people are allergic to penicillin, and this does not mean that we should not use it in treatment<bold>. </bold></p>
        <p>
          <italic>Therefore, before use, one must do the Allergy Test: </italic>
          <italic>the</italic>
          <italic> Test as Follow</italic>
        </p>
        <p>Withdraw a small amount of the solution that we will use in the treatment, about 0.2 ml, and then inject it    S/C in skin of the arm; thenwait for one hour<bold>: </bold>If the           injection site showed severe effects of the injection,            similar to burning with hot water with itching in the eyes and body, then this person has a natural allergy to the drug.<xref ref-type="bibr" rid="ridm1850016452">4</xref></p>
        <p>
          <italic>How do we Treat Allergies if it Appears during Treatment?</italic>
        </p>
        <p>1. Inject the patient with Avil intramuscularly (I/M)</p>
        <p>2. Allergy symptoms will go away immediately</p>
      </sec>
      <sec id="idm1850253276">
        <title>Bee Venom Possibilities to Use In People Who are Allergic but Need it</title>
        <p>Yes, under rigorous supervision, but with a           modification in the route of administration, in this case allergic patient will receive treatment through food and avoiding parenteral route of bee venom injections.              Especially in treatment of cancer and chronic viral             infections, wherever, other treatments are not effective or unavailable, or expensive, etc.<xref ref-type="bibr" rid="ridm1849778420">5</xref></p>
        <p>How to prepare the medicine through food and drink</p>
        <p>1. Add 0.5 gram whole (crude) Bee Venom / kilogram of natural bee honey: the doses are determined               according to each case, ingestion route </p>
        <p>2. Add one gram / three liters of clean drinking water: the doses are determined according to each case;           ingestion route</p>
        <p>This method is suitable for all people in general &amp; in order to improve immunity and prevent diseases</p>
        <p>- To treat diseases caused by various viruses</p>
        <p>- Treat Lyme disease</p>
        <p>- Patients who receive local injections of the same drug</p>
        <p>- Smokers and other groups at risk of developing cancer as a result of genetic or acquired factors</p>
        <p>- Other uses; arthritis, chronic inflammations, etc.</p>
        <p>So that; doctor must be familiar with the                 properties of bee venom in order to be creative and           benefit from the tremendous therapeutic capabilities of bee venom.</p>
      </sec>
      <sec id="idm1850248884">
        <title>Bee Venom is Consumed in the Presence of Diseases Bio Agents in Vivo</title>
        <p>It means that if bee venom injected into cancer lesions or lesions of viral infections (for example; herpes virus or pox virus) the bee venom will be used up during the process of eliminating cancerous cells or viral infected cells; bee venom will kill the causative agent and destruct the infected cells. </p>
        <p>It also means that if bee venom injected into healthy skin or tissue, it will not be completely consumed, which means that the determination of doses depends on the condition of the recipient of bee venom treatment, and the doses of bee venom must be adjusted according to this fact, taking into consideration that patient must be under medical observation and adjust our doses according to what we notice with naked eye.</p>
        <p>Therefore, the doctor must receive adequate           laboratory and experimental training before using this method, or it is urgent they can perform it under the         supervision of the experts in this drug (the duty of the clinical pharmacist).<xref ref-type="bibr" rid="ridm1849776188">6</xref></p>
        <p>
          <italic>Cationic Peptides (Mellitin)</italic>
          <sup>
            <italic> +</italic>
          </sup>
          <sup>
            <italic>ve</italic>
          </sup>
          <italic> Role in Mechanism of Action of Bee Venom as Anticancer and Antiviral in Vivo</italic>
        </p>
        <p>There is a negative charge on cancer cells, viral infected cells, diseased cells, and generally any cells that contain toxins or damage, and viruses are carrying             negative charge even when it is outside the living body.</p>
        <p>In the components of bee venom, there is a            substance melittin, which carries a positive charge, so the mode of action of bee venom in the treatment of cancer is similar to cationic peptides effects, that is, bee venom break down and attack negatively charged cancer cells. However, healthy tissue does not carry electric charges. </p>
        <p>The role that the herpes virus is likely to play in increasing the severity of cancerous diseases, worsen the conditions.</p>
        <p>Why do we assume this: because our observation in examining samples by electron microscope shows the presence of different types of herpes virus in tissues that suffer from diseases caused by other major causal factors.</p>
        <p>Also, herpes viruses are opportunistic viruses that strike the body whose immunity is weakened for any              reason. Therefore, the role of this virus must be                         neutralized when you planning to treat a cancer patient.</p>
        <p>Fortunately, bee venom is a powerful antiviral, and thus we hit three birds with one stone, that is, we kill cancer cells, kill opportunistic viruses, and improve tissue immunity to participate in the fight against cancer and get rid of toxic exudates more efficiently.</p>
        <p>
          <italic>Important Notes (Vital):</italic>
        </p>
        <p>1. You must rely on the newly produced, fresh prepared, whole bee venom. </p>
        <p>2. You must rely on bee venom free from human                 intervention and do not  separate its components from each other, because the components of bee               venom cooperate with each other in synergistic                actions to cause the pharmacological effect of the drug.</p>
        <p>3. It is forbidden to smell bee venom </p>
        <p>4. It is forbidden to rub the eye while the hand or the tool has the residue of the bee venom</p>
        <p>5. the face mask should be placed on the nose and the eyes should be covered with transparent glasses while dealing with bee venom</p>
        <p>6. Always have (Avil) syringe ready for use in case of any allergic symptoms</p>
      </sec>
    </sec>
  </body>
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