<?xml version="1.0" encoding="utf8"?>
 <!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.0 20120330//EN" "http://jats.nlm.nih.gov/publishing/1.0/JATS-journalpublishing1.dtd"> <article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" article-type="research-article" dtd-version="1.0" xml:lang="en">
  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">JAR</journal-id>
      <journal-title-group>
        <journal-title>Journal of Agronomy Research</journal-title>
      </journal-title-group>
      <issn pub-type="epub">2639-3166</issn>
      <publisher>
        <publisher-name>Open Access Pub</publisher-name>
        <publisher-loc>United States</publisher-loc>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="publisher-id">JAR-19-2691</article-id>
      <article-id pub-id-type="doi">10.14302/issn.2639-3166.jar-19-2691</article-id>
      <article-categories>
        <subj-group>
          <subject>research-article</subject>
        </subj-group>
      </article-categories>
      <title-group>
        <article-title>Rice Yield Advances Under Precision Agriculture: a Farm Lesson</article-title>
        <alt-title alt-title-type="running-head">precision agriculture in a rice farm</alt-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author">
          <name>
            <surname>Giuseppe</surname>
            <given-names>Sarasso</given-names>
          </name>
          <xref ref-type="aff" rid="idm1842823340">1</xref>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Roberto</surname>
            <given-names>Sarasso</given-names>
          </name>
          <xref ref-type="aff" rid="idm1842823988">2</xref>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Antonio</surname>
            <given-names>Finassi</given-names>
          </name>
          <xref ref-type="aff" rid="idm1842823340">1</xref>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Giorgio</surname>
            <given-names>Masoero</given-names>
          </name>
          <xref ref-type="aff" rid="idm1842823340">1</xref>
          <xref ref-type="aff" rid="idm1842821972">*</xref>
        </contrib>
      </contrib-group>
      <aff id="idm1842823340">
        <label>1</label>
        <addr-line>Accademia di Agricoltura di Torino (TO)</addr-line>
      </aff>
      <aff id="idm1842823988">
        <label>2</label>
        <addr-line>Azienda Agricola Palestro, Olcenengo (VC)</addr-line>
      </aff>
      <aff id="idm1842821972">
        <label>*</label>
        <addr-line>Corresponding author</addr-line>
      </aff>
      <contrib-group>
        <contrib contrib-type="editor">
          <name>
            <surname>Abubaker</surname>
            <given-names>Haroun Mohamed Adam</given-names>
          </name>
          <xref ref-type="aff" rid="idm1842653908">1</xref>
        </contrib>
      </contrib-group>
      <aff id="idm1842653908">
        <label>1</label>
        <addr-line>Department of Crop Science (Agronomy), College of Agriculture, Bahri University- Alkadaru- Khartoum -Sudan.</addr-line>
      </aff>
      <author-notes>
        <corresp>
    
    Giorgio Masoero, <addr-line>Accademia di Agricoltura di Torino, Via A. </addr-line><addr-line>Doria</addr-line><addr-line> 10, 10123, Torino, Italy</addr-line>. E-mail: <email>giorgioxmasoero@gmail.com</email></corresp>
        <fn fn-type="conflict" id="idm1842808004">
          <p>The authors have declared that no competing interests exist.</p>
        </fn>
      </author-notes>
      <pub-date pub-type="epub" iso-8601-date="2019-03-08">
        <day>08</day>
        <month>03</month>
        <year>2019</year>
      </pub-date>
      <volume>1</volume>
      <issue>4</issue>
      <fpage>10</fpage>
      <lpage>21</lpage>
      <history>
        <date date-type="received">
          <day>26</day>
          <month>02</month>
          <year>2019</year>
        </date>
        <date date-type="accepted">
          <day>05</day>
          <month>03</month>
          <year>2019</year>
        </date>
        <date date-type="online">
          <day>08</day>
          <month>03</month>
          <year>2019</year>
        </date>
      </history>
      <permissions>
        <copyright-statement>© </copyright-statement>
        <copyright-year>2019</copyright-year>
        <copyright-holder>Giuseppe Sarasso, et al.</copyright-holder>
        <license xlink:href="http://creativecommons.org/licenses/by/4.0/" xlink:type="simple">
          <license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p>
        </license>
      </permissions>
      <self-uri xlink:href="http://openaccesspub.org//jar/article/1030">This article is available from http://openaccesspub.org//jar/article/1030</self-uri>
      <abstract>
        <p>In precision agriculture (PA) fertilizing, based on soil testing, production maps and crop nitrogen (N) demand, is the key to maximizing yields and tempering fertilizer costs. A trend study has considered the output / input relationships performed on a farm  that has progressively adapted to PA procedures over two decades. The evolutions of the variability parameters of yield, comprising the repeatability coefficient of repeated plots, the vegetative vigour (NDRE) at the panicle initiation (pi) stage, and the nitrogen utilization efficiency (NUE) were monitored and compared by means of mixed linear models over a six-year period, after the variable nitrogen (N) fertilization rate (VNFR) had been enlarged to the whole 230 ha of one farm. At pi key fertilization stage, a corrective dose was applied by tacking the correlation between Npi and the measured NDRE in strong negative mode. The evolution of the yield, for the 2012-2017 interval, based on 1165 ha<sup>-1</sup> parcel-data, showed a significant yearly increase of 2.3% more than the regional trend (+0.5%).  The variability parameters of the yield, that is, the standard deviation (+7.3%), range (+7.1%), coefficient of variation (+5.4%) and maximum (+2.1%) were enhanced over the years, but the minimum remained stable.  The repeatability of the parcel yield generally appeared low (r = +0.31), but it tended to increase by 8.3% year<sup>-1</sup> (P = 0.018). At the same time, the vegetational vigour also showed significant increases of the NDRE means (+3.0%) as well as of the maximum (+0.8%), but also large oscillations in the standard deviation and in the coefficient of variation.  No significant regression of the NDRE on the coefficient of variation of the yield was established. The favorable increase in yield was found to be independent of the distributed N-total. A strong negative correlation (imposed) between N-pi and NDRE (-0.90) and a negative correlation with production were observed for a sample field (but in the area of maximum production). It is recommended that a partial correlation between Yield and N-tot should be considered in the I /O features for a parity of NDRE, which apparently decreases the negativity of the relationship. In short: with the same total input of N, the PA increased the yield, but also its variability – and it did not reduce the variability as predicted by the theory - by strengthening the repeatability. This is an evidence that in many of the parcels with minimum yield the limiting factors cannot  be referred to the N availability. </p>
      </abstract>
      <kwd-group>
        <kwd>precision agriculture</kwd>
        <kwd>rice</kwd>
        <kwd>variable nitrogen fertilizer rate</kwd>
        <kwd>NDRE</kwd>
        <kwd>yield evolution</kwd>
        <kwd>standard deviation</kwd>
        <kwd>yield repeatability</kwd>
        <kwd>correlation</kwd>
        <kwd>partial correlation</kwd>
        <kwd>NUE.</kwd>
      </kwd-group>
      <counts>
        <fig-count count="12"/>
        <table-count count="6"/>
        <page-count count="12"/>
      </counts>
    </article-meta>
  </front>
  <body>
    <sec id="idm1842657724" sec-type="intro">
      <title>Introduction</title>
      <p>After long and detailed studies focused on production models developed in universities <xref ref-type="bibr" rid="ridm1843298828">1</xref>,<xref ref-type="bibr" rid="ridm1843295444">2</xref>,<xref ref-type="bibr" rid="ridm1843303484">3</xref>and adopted by extension services <xref ref-type="bibr" rid="ridm1843401636">4</xref>,<xref ref-type="bibr" rid="ridm1843158372">5</xref> it is now time to advance precision agriculture (PA), resulting from the developments made in the mechanical agricultural industry and from the work of scientists who produced a body of 17,756 scientific publications, indexed in  the Scopus database from 2000 to 2016, throughout the world <xref ref-type="bibr" rid="ridm1843157364">6</xref>. The PA mainly involves: reduction of overlaps of fertilizers and pesticides during the distribution; soil tillage managements; precision in the dosage of the inputs for the best production. The theoretical potentialities of the various operating instruments now available are challenged by the “big data” generated from the fields as well as from the environment. However, the complexity of the large number of factors, and the multiple interference between their forcing activities that affect production are incomparably less “precise” than the PA definition suggests. Experimenting with a single sensor (<xref ref-type="fig" rid="idm1842815084">Figure 1</xref>) is relatively simple, but it is not straightforward to include the results in the complex system of factors that influence productivity, many of which are unknown or uncontrollable. </p>
      <fig id="idm1842815084">
        <label>Figure 1.</label>
        <caption>
          <title> The articulated mechanic support of the vigor sensor, to compare the different responses at three inclinations, with best result when inclined 16° from the horizontal. </title>
        </caption>
        <graphic xlink:href="images/image1.jpeg" mime-subtype="jpeg"/>
      </fig>
      <p>The progress in yield raising and homogenizing by use of the fertilization maps may be quickly achieved at the beginning of the process <xref ref-type="bibr" rid="ridm1843401636">4</xref>,<xref ref-type="bibr" rid="ridm1843158372">5</xref>and this fact is exemplified in the <xref ref-type="fig" rid="idm1842813500">Figure 2</xref>.</p>
      <fig id="idm1842813500">
        <label>Figure 2.</label>
        <caption>
          <title> The improvement of the evenness of yields achieved between 2001 (mean 7.94 t ha-1) and 2007 (mean 8.97 t ha-1) modulating the rates of organic fertilizer, based on yield maps.</title>
        </caption>
        <graphic xlink:href="images/image2.jpeg" mime-subtype="jpeg"/>
      </fig>
      <p>Since the PA is welcome in rice cultivation, in this work we aimed to study in retrospect the productive consequences of its application on a PA Italian rice farm, oriented to obtain maximum yields. In this paper we are concerned in featuring the trends in the variability parameters, obtaining some lessons from fields.</p>
      <sec id="idm1842643580">
        <title>Experimental Procedure</title>
        <p>A trend study has considered the output / input relationships performed on a farm  that has progressively adapted to PA procedures over two decades. The evolutions of the variability parameters of yield, comprising  the repeatability coefficient of repeated plots, the vegetative vigour (NDRE) at the panicle initiation (pi) stage, and the nitrogen utilization efficiency (NUE) were monitored and compared by means of mixed linear models over a six-year period, after the variable nitrogen (N) fertilization rate (VNFR) had been enlarged to the whole farm.</p>
      </sec>
    </sec>
    <sec id="idm1842643436" sec-type="materials">
      <title>Material and Methods</title>
      <p>The PA experience started in 1998 with a harvester which was equipped with a yield meter, and then with a yield monitor, a satellite receiver and a light bar to assist manual drive. The farm now spreads fertilizers using a VRT technology, following prescription maps or real time signals of vigor sensors using  an OptRx by AgLeader (<xref ref-type="fig" rid="idm1842783340">Figure 3</xref>), which reads both NDVI (wavelengths of 670 nm and 780 nm) and NDRE (730 nm and 780 nm). </p>
      <fig id="idm1842783340">
        <label>Figure 3.</label>
        <caption>
          <title> The tractor’s cab full of monitors and cables, due to the lack of communication between monitors and sensors of different brands</title>
        </caption>
        <graphic xlink:href="images/image3.jpeg" mime-subtype="jpeg"/>
      </fig>
      <p>Before N-pi administration, a drone was used to assess the mean and probable range of the NDRE readings of the sensor, and to fix the thresholds. Following an RTK signal, herbicides are sprayed by a VRT sprayer, which controls the opening and closing of the nozzles lead from a GPS signal, and yield maps are obtained by an harvester equipped with an AgLeader yield meter. All the technology had to be adapted to the soil (<xref ref-type="table" rid="idm1842789604">Table 1</xref>). The name of the farm, “Palestro” (GPS data of the farm buildings: WGS 84 zone 32 N Lat 45,371488 – Lon 8,298165), comes from the Latin “<italic>Palustris</italic>”, which means Swampy. All the soil, that is, 249 ha, of which 230 ha are cultivated, is silty-clay, and is flooded from April to August. Moreover, a rice monoculture has been grown on this farm since 1965.</p>
      <table-wrap id="idm1842789604">
        <label>Table 1.</label>
        <caption>
          <title> Range in characteristics of soil samples from 14 fields in 2012-16, at a depth of 30 cm.</title>
        </caption>
        <table rules="all" frame="box">
          <tbody>
            <tr>
              <td>Items</td>
              <td>Units</td>
              <td>Worst</td>
              <td>Best</td>
            </tr>
            <tr>
              <td>Average Yields</td>
              <td>t ha<sup>-1</sup></td>
              <td>7.30</td>
              <td>8.29</td>
            </tr>
            <tr>
              <td>Gravel (&gt;2mm)</td>
              <td>mg kg dry sub.<sup>-1</sup>.</td>
              <td>12.57</td>
              <td>16.00</td>
            </tr>
            <tr>
              <td>Sand (2-0.5 mm)</td>
              <td>mg kg dry sub.<sup> -1</sup></td>
              <td>312.71</td>
              <td>332.71</td>
            </tr>
            <tr>
              <td>Silt (0.5-0.02 mm)</td>
              <td>mg kg dry sub.<sup> -1</sup></td>
              <td>582.29</td>
              <td>565.14</td>
            </tr>
            <tr>
              <td>Clay (&lt;0.02 mm)</td>
              <td>mg kg dry sub.<sup> -1</sup></td>
              <td>105.00</td>
              <td>102.14</td>
            </tr>
            <tr>
              <td>pH H<sub>2</sub>O</td>
              <td>pH units</td>
              <td>5.70</td>
              <td>5.77</td>
            </tr>
            <tr>
              <td>pH CaCl2</td>
              <td>pH units</td>
              <td>4.97</td>
              <td>5.01</td>
            </tr>
            <tr>
              <td>CaCO<sub>3</sub> TOT</td>
              <td>mg kg dry sub.<sup> -1</sup></td>
              <td>0.00</td>
              <td>0.00</td>
            </tr>
            <tr>
              <td>CaCO<sub>3</sub> ACTIVE</td>
              <td>mg kg dry sub.<sup> -1</sup></td>
              <td>0.00</td>
              <td>0.00</td>
            </tr>
            <tr>
              <td>Organic Matter (calculated)</td>
              <td>mg kg dry sub.<sup> -1</sup></td>
              <td>22.57</td>
              <td>22.00</td>
            </tr>
            <tr>
              <td>Organic C (Dumas)</td>
              <td>mg kg dry sub.<sup> -1</sup></td>
              <td>13.11</td>
              <td>12.76</td>
            </tr>
            <tr>
              <td>N TOT</td>
              <td>mg kg dry sub.<sup> -1</sup></td>
              <td>1.30</td>
              <td>1.24</td>
            </tr>
            <tr>
              <td>C/N rate</td>
              <td> </td>
              <td>9.91</td>
              <td>10.23</td>
            </tr>
            <tr>
              <td>Cationic exchange capacity</td>
              <td>meq 100g dry sub.<sup> -1</sup></td>
              <td>13.46</td>
              <td>13.11</td>
            </tr>
            <tr>
              <td>Exchangeable Ca</td>
              <td>meq 100g dry sub.<sup> -1</sup></td>
              <td>4.43</td>
              <td>4.22</td>
            </tr>
            <tr>
              <td>Exchangeable Mg</td>
              <td>meq 100g dry sub.<sup> -1</sup></td>
              <td>0.86</td>
              <td>0.82</td>
            </tr>
            <tr>
              <td>Exchangeable K</td>
              <td>meq 100g dry sub.<sup> -1</sup></td>
              <td>0.24</td>
              <td>0.20</td>
            </tr>
            <tr>
              <td>Exchangeable Na</td>
              <td>meq 100g dry sub.<sup> -1</sup></td>
              <td>0.04</td>
              <td>0.04</td>
            </tr>
            <tr>
              <td>Basis saturation</td>
              <td>%</td>
              <td>41.29</td>
              <td>40.14</td>
            </tr>
            <tr>
              <td>Ca/Mg  rate</td>
              <td> </td>
              <td>5.17</td>
              <td>5.20</td>
            </tr>
            <tr>
              <td>Mg/K   rate</td>
              <td> </td>
              <td>3.87</td>
              <td>3.99</td>
            </tr>
            <tr>
              <td>Assimilable P</td>
              <td>mg kg dry sub.<sup> -1</sup></td>
              <td>27.00</td>
              <td>29.86</td>
            </tr>
          </tbody>
        </table>
      </table-wrap>
      <p>Yield data from each 19x19m2 mesh plot have been elaborated for the 2012-17 interval (No. 1165 ha<sup>-1</sup>). Yield repeatability was calculated as a Pearson correlation of the plot-yields within field between consecutive  years. Several mixed models (PROC MIXED by SAS 9.0), concerning the yield and NDRE, were applied to the statistical parameters of the fields, whereby the effects of the field or cultivar were considered fixed and the year was taken as the random factor in order to estimate the linear trend. Conversely, when the years were considered fixed, and the fields were considered as the random factor, a least squares solution was found. The covariation of the variability between the different traits was enhanced by fitting a linear or non-linear regression of the estimates. A Friedman’s test for paired samples was used to compare the yield averages of the farm <italic>vs</italic>. the regional means <xref ref-type="bibr" rid="ridm1843160532">7</xref>. In a sample field, over a period of two years, the total N (N-tot), and  N divided into N-<italic>pre-</italic>pi and N-pi, was related to the yield by developing a partial correlation with NDRE.    </p>
      <p>Place: r12; r13 and r23 then r12.3 = (r12 – r13 * r23) / ((1-r13^2)^.5* (1-r23^2)^.5)).   </p>
      <p>Place: regression b1/2 = covariance12 / stdev2 ^2; regression b2/1 = covariance 12 / stdev1 ^2 then (b1 * b2) = r12^2.</p>
      <sec id="idm1842580148">
        <title>Results</title>
        <sec id="idm1842581228">
          <title>Global Yield, N and NUE Trends Over the Years. </title>
          <p>A yearly increase of +2.3% more than the +0.5% of the regional features (<xref ref-type="table" rid="idm1842693804">Table 2</xref>) pointed out a substantial response from PF practices. For an N supply increment of +0.9%, the NUE increase was +1.7%. The trends appeared nonlinear for all the considered average parameters (<xref ref-type="fig" rid="idm1842694596">Figure 4</xref>). It should be noted that the average yields of the farm were 17% above the regional means (P 001).</p>
          <fig id="idm1842694596">
            <label>Figure 4.</label>
            <caption>
              <title> Nonlinear trends of the total N and Yield over a six year period, compared with the production of NW Italy NW.</title>
            </caption>
            <graphic xlink:href="images/image4.jpg" mime-subtype="jpg"/>
          </fig>
          <table-wrap id="idm1842693804">
            <label>Table 2.</label>
            <caption>
              <title> N-Total, yield and NUE gross averages over a six year period, compared with the production in NW Italy.</title>
            </caption>
            <table rules="all" frame="box">
              <tbody>
                <tr>
                  <td>Year</td>
                  <td>N-total</td>
                  <td>Yield</td>
                  <td>NUE</td>
                  <td>NW Italy yield</td>
                </tr>
                <tr>
                  <td> </td>
                  <td>kg ha<sup>-1</sup></td>
                  <td>t ha<sup>-1</sup></td>
                  <td>kg N kg<sup>-1</sup></td>
                  <td>t ha<sup>-1</sup></td>
                </tr>
                <tr>
                  <td>2012</td>
                  <td>165.0</td>
                  <td>7.47</td>
                  <td>45.30</td>
                  <td>6.80</td>
                </tr>
                <tr>
                  <td>2013</td>
                  <td>167.0</td>
                  <td>7.62</td>
                  <td>45.63</td>
                  <td>6.63</td>
                </tr>
                <tr>
                  <td>2014</td>
                  <td>165.0</td>
                  <td>7.65</td>
                  <td>46.38</td>
                  <td>6.44</td>
                </tr>
                <tr>
                  <td>2015</td>
                  <td>160.8</td>
                  <td>7.70</td>
                  <td>47.88</td>
                  <td>6.67</td>
                </tr>
                <tr>
                  <td>2016</td>
                  <td>162.2</td>
                  <td>8.21</td>
                  <td>50.60</td>
                  <td>6.77</td>
                </tr>
                <tr>
                  <td>2017</td>
                  <td>184.3</td>
                  <td>8.36</td>
                  <td>45.39</td>
                  <td>6.92</td>
                </tr>
                <tr>
                  <td>Mean</td>
                  <td>167.4</td>
                  <td>7.84 a</td>
                  <td>46.9</td>
                  <td>6.70 b</td>
                </tr>
                <tr>
                  <td>Trend\year</td>
                  <td>1.58</td>
                  <td>1.63</td>
                  <td>0.8</td>
                  <td>0.8</td>
                </tr>
                <tr>
                  <td>Linear trend %</td>
                  <td>0.9%</td>
                  <td>2.3%</td>
                  <td>1.7%</td>
                  <td>0.5%</td>
                </tr>
                <tr>
                  <td>P.value linear</td>
                  <td>0.053</td>
                  <td>0.009</td>
                  <td>0.063</td>
                  <td>0.213</td>
                </tr>
                <tr>
                  <td>P.value quadratic</td>
                  <td>0.033</td>
                  <td>0.067</td>
                  <td>0.214</td>
                  <td> </td>
                </tr>
                <tr>
                  <td>P.value cubic</td>
                  <td>0.023</td>
                  <td> </td>
                  <td>0.192</td>
                  <td> </td>
                </tr>
              </tbody>
            </table>
            <table-wrap-foot>
              <fn id="idm1842536196">
                <label/>
                <p>a&gt;b, P =0.01</p>
              </fn>
            </table-wrap-foot>
          </table-wrap>
        </sec>
        <sec id="idm1842535188">
          <title>Yield Trend and Variability</title>
          <p>The statistics of the yield of the single plots confirmed the +2.3% year<sup>-1 </sup>trend(<xref ref-type="table" rid="idm1842693804">Table 2</xref>), but a very highly significant regression coefficient was observed (P = 0.0002). The variability of the yield appeared to increase over the years, in terms of the standard deviation (+7.3%), coefficient of variation (+5.4%) and range (+7.1%), and only the minimum was stable.  A nonlinear shape of the standard deviation is apparent in <xref ref-type="fig" rid="idm1842638308">Figure 5</xref>, where a positive and a negative peak can be observed to have affected the performances in 2013 and 2014, respectively.  The repeatability of the yield was below 0.10 in 2012, but after a yearly increase of some 8.3%, it reached a value of 0.35 in 2017. As can be observed in <xref ref-type="fig" rid="idm1842637300">Figure 6</xref> a co-evolution of the coefficient of variation and of the repeatability is apparent, with a departure from linearity in 2014. (<xref ref-type="table" rid="idm1842617132">Table 3</xref>)</p>
          <fig id="idm1842638308">
            <label>Figure 5.</label>
            <caption>
              <title> Trend of yield statistics over the years.</title>
            </caption>
            <graphic xlink:href="images/image5.jpg" mime-subtype="jpg"/>
          </fig>
          <fig id="idm1842637300">
            <label>Figure 6.</label>
            <caption>
              <title> Co-evolution of the coefficient of variation with the plot repeatability of yield.</title>
            </caption>
            <graphic xlink:href="images/image6.jpg" mime-subtype="jpg"/>
          </fig>
          <table-wrap id="idm1842617132">
            <label>Table 3.</label>
            <caption>
              <title> Statistics of the plot yield over the years (No. 398 820).</title>
            </caption>
            <table rules="all" frame="box">
              <tbody>
                <tr>
                  <td> </td>
                  <td>Mean</td>
                  <td>St.dev</td>
                  <td>Coef.var</td>
                  <td>Max</td>
                  <td>Min</td>
                  <td>Range</td>
                  <td>Repeatability</td>
                </tr>
                <tr>
                  <td>2012</td>
                  <td>7.48</td>
                  <td>0.85</td>
                  <td>0.10</td>
                  <td>8.91</td>
                  <td>5.19</td>
                  <td>3.58</td>
                  <td>0.183</td>
                </tr>
                <tr>
                  <td>2013</td>
                  <td>7.62</td>
                  <td>1.05</td>
                  <td>0.12</td>
                  <td>9.24</td>
                  <td>4.94</td>
                  <td>4.13</td>
                  <td>0.273</td>
                </tr>
                <tr>
                  <td>2014</td>
                  <td>7.65</td>
                  <td>0.82</td>
                  <td>0.09</td>
                  <td>8.86</td>
                  <td>5.16</td>
                  <td>3.56</td>
                  <td>0.445</td>
                </tr>
                <tr>
                  <td>2015</td>
                  <td>7.70</td>
                  <td>1.02</td>
                  <td>0.10</td>
                  <td>8.93</td>
                  <td>4.62</td>
                  <td>4.28</td>
                  <td>0.284</td>
                </tr>
                <tr>
                  <td>2016</td>
                  <td>8.21</td>
                  <td>1.17</td>
                  <td>0.12</td>
                  <td>9.61</td>
                  <td>4.94</td>
                  <td>4.67</td>
                  <td>0.325</td>
                </tr>
                <tr>
                  <td>2017</td>
                  <td>8.36</td>
                  <td>1.26</td>
                  <td>0.13</td>
                  <td>10.01</td>
                  <td>4.84</td>
                  <td>5.20</td>
                  <td>0.370</td>
                </tr>
                <tr>
                  <td>Mean</td>
                  <td>7.84</td>
                  <td>1.03</td>
                  <td>0.11</td>
                  <td>9.26</td>
                  <td>4.95</td>
                  <td>4.24</td>
                  <td>0.313</td>
                </tr>
                <tr>
                  <td>P.value Means</td>
                  <td>0.0073</td>
                  <td>0.0004</td>
                  <td>0.0247</td>
                  <td>0.0212</td>
                  <td>0.7794</td>
                  <td>0.0032</td>
                  <td>0.002</td>
                </tr>
                <tr>
                  <td>Trend year<sup>-1</sup></td>
                  <td>0.179</td>
                  <td>0.075</td>
                  <td>0.006</td>
                  <td>0.191</td>
                  <td>-0.065</td>
                  <td>0.299</td>
                  <td>0.026</td>
                </tr>
                <tr>
                  <td>Trend %</td>
                  <td>2.3%</td>
                  <td>7.3%</td>
                  <td>5.4%</td>
                  <td>2.1%</td>
                  <td>-1.3%</td>
                  <td>7.1%</td>
                  <td>8.3%</td>
                </tr>
                <tr>
                  <td>P.value linear</td>
                  <td>0.0002</td>
                  <td>0.0003</td>
                  <td>0.0164</td>
                  <td>0.0071</td>
                  <td>0.3633</td>
                  <td>0.0003</td>
                  <td>0.0184</td>
                </tr>
                <tr>
                  <td>P.value quadratic</td>
                  <td> </td>
                  <td>0.224</td>
                  <td>0.147</td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td>0.200</td>
                </tr>
                <tr>
                  <td>R<xref ref-type="bibr" rid="ridm1843295444">2</xref> adj</td>
                  <td>0.824</td>
                  <td>0.525</td>
                  <td>0.463</td>
                  <td>0.494</td>
                  <td>0.164</td>
                  <td>0.712</td>
                  <td>0.125</td>
                </tr>
              </tbody>
            </table>
          </table-wrap>
        </sec>
        <sec id="idm1842471196">
          <title>NDRE Trend and Variability</title>
          <p>The NDRE trend grew by +3.0% year<sup>-1</sup>                       (P =0.044), with significantly different annual values               (P =0.000; <xref ref-type="table" rid="idm1842505484">Table 4</xref> and <xref ref-type="fig" rid="idm1842560660">Figure 7</xref>).</p>
          <fig id="idm1842560660">
            <label>Figure 7.</label>
            <caption>
              <title>  Trend of the NDRE statistics over a six year period.</title>
            </caption>
            <graphic xlink:href="images/image7.jpg" mime-subtype="jpg"/>
          </fig>
          <table-wrap id="idm1842505484">
            <label>Table 4.</label>
            <caption>
              <title>  Year and trend effects on the vegetation vigour NDRE statistics (No. 46 from 289  plots ha-1 of the elementary observations of two OptRx sensors that followed parallel paths in the fields) .</title>
            </caption>
            <table rules="all" frame="box">
              <tbody>
                <tr>
                  <td>Year</td>
                  <td>Mean <xref ref-type="table-fn" rid="idm1842425380">a</xref></td>
                  <td>St. dev</td>
                  <td>Coef. Var <xref ref-type="table-fn" rid="idm1842425380">a</xref></td>
                  <td>Max</td>
                  <td>Min</td>
                  <td>Range</td>
                </tr>
                <tr>
                  <td>2012</td>
                  <td>0.254</td>
                  <td>0.032</td>
                  <td>0.136</td>
                  <td>0.319</td>
                  <td>0.194</td>
                  <td>0.157</td>
                </tr>
                <tr>
                  <td>2013</td>
                  <td>0.295</td>
                  <td>0.033</td>
                  <td>0.140</td>
                  <td>0.375</td>
                  <td>0.227</td>
                  <td>0.215</td>
                </tr>
                <tr>
                  <td>2014</td>
                  <td>0.311</td>
                  <td>0.031</td>
                  <td>0.100</td>
                  <td>0.361</td>
                  <td>0.231</td>
                  <td>0.150</td>
                </tr>
                <tr>
                  <td>2015</td>
                  <td>0.316</td>
                  <td>0.037</td>
                  <td>0.119</td>
                  <td>0.370</td>
                  <td>0.212</td>
                  <td>0.214</td>
                </tr>
                <tr>
                  <td>2016</td>
                  <td>0.290</td>
                  <td>0.032</td>
                  <td>0.113</td>
                  <td>0.347</td>
                  <td>0.195</td>
                  <td>0.163</td>
                </tr>
                <tr>
                  <td>2017</td>
                  <td>0.317</td>
                  <td>0.035</td>
                  <td>0.115</td>
                  <td>0.354</td>
                  <td>0.236</td>
                  <td>0.181</td>
                </tr>
                <tr>
                  <td>Mean</td>
                  <td>0.297</td>
                  <td>0.033</td>
                  <td>0.121</td>
                  <td>0.354</td>
                  <td>0.216</td>
                  <td>0.180</td>
                </tr>
                <tr>
                  <td>P.value  Mean</td>
                  <td>0.000</td>
                  <td>0.032</td>
                  <td>0.290</td>
                  <td>0.027</td>
                  <td>0.195</td>
                  <td>0.027</td>
                </tr>
                <tr>
                  <td>Trend year<sup>-1</sup></td>
                  <td>0.009</td>
                  <td>0.001</td>
                  <td>-0.005</td>
                  <td>0.003</td>
                  <td>0.003</td>
                  <td>0.001</td>
                </tr>
                <tr>
                  <td>Trend %</td>
                  <td>3.0%</td>
                  <td>3.0%</td>
                  <td>-4.1%</td>
                  <td>0.8%</td>
                  <td>1.4%</td>
                  <td>0.6%</td>
                </tr>
                <tr>
                  <td>P.value</td>
                  <td>0.044</td>
                  <td>0.240</td>
                  <td>0.833</td>
                  <td>0.054</td>
                  <td>0.355</td>
                  <td>0.077</td>
                </tr>
              </tbody>
            </table>
            <table-wrap-foot>
              <fn id="idm1842425380">
                <label>a</label>
                <p>: correlation (mean, coef. var)  =-0.33</p>
              </fn>
            </table-wrap-foot>
          </table-wrap>
          <p>The SD spanned the years (P =0.032) and rose by  +3% (not significant) with an important rise in 2015.</p>
          <p>The coefficient of variation did not fluctuate over the years, but highlighted a descending parabolic trend (P =0.05.</p>
          <p>The Minimum remained stable over the years, while the range and the maximum oscillated (P =0.027), but tended to increase by 1.2% year<sup>-1</sup>, following a nonlinear shape.</p>
        </sec>
        <sec id="idm1842422932">
          <title>Yield and NDRE Co-Evolution</title>
          <p>The co-evolution of the two variables was apparently null (<xref ref-type="fig" rid="idm1842440212">Figure 8</xref>). However, when two anomalous years, namely 2012 (excessively   homogenous) and 2017 (excessively variable) were excluded, the regression clearly appeared negative: a higher NDRE led to a less variable production. </p>
          <fig id="idm1842440212">
            <label>Figure 8.</label>
            <caption>
              <title> Yearly regression of the coefficient of variation of the yield on the NDRE values, with outliers for the two years.</title>
            </caption>
            <graphic xlink:href="images/image8.jpg" mime-subtype="jpg"/>
          </fig>
        </sec>
        <sec id="idm1842422572">
          <title>NDRE in the Cultivars</title>
          <p>The vegetation vigour NDRE statistics of fifteen cultivars grown in Italy are reported in <xref ref-type="table" rid="idm1842439852">Table 5</xref>. The <italic>Selenio</italic> records were outliers. </p>
          <table-wrap id="idm1842439852">
            <label>Table 5.</label>
            <caption>
              <title> The vegetation vigour NDRE cultivar statistics over the six year period (No. 46 from 289 averages ha-1 of the elementary observations of two OptRx sensors that followed parallel paths in the fields)</title>
            </caption>
            <table rules="all" frame="box">
              <tbody>
                <tr>
                  <td>Cultivar</td>
                  <td>Mean</td>
                  <td>St. dev</td>
                  <td>Max</td>
                  <td>Min</td>
                  <td>Range</td>
                  <td>Coef. variation</td>
                </tr>
                <tr>
                  <td>ALLEGRO</td>
                  <td>0.201</td>
                  <td>0.025</td>
                  <td>0.283</td>
                  <td>0.137</td>
                  <td>0.183</td>
                  <td>13%</td>
                </tr>
                <tr>
                  <td>CARNISE_</td>
                  <td>0.262</td>
                  <td>0.024</td>
                  <td>0.341</td>
                  <td>0.168</td>
                  <td>0.141</td>
                  <td>10%</td>
                </tr>
                <tr>
                  <td>RONALDO</td>
                  <td>0.285</td>
                  <td>0.033</td>
                  <td>0.381</td>
                  <td>0.142</td>
                  <td>0.202</td>
                  <td>12%</td>
                </tr>
                <tr>
                  <td>LEONARDO</td>
                  <td>0.299</td>
                  <td>0.029</td>
                  <td>0.398</td>
                  <td>0.231</td>
                  <td>0.199</td>
                  <td>10%</td>
                </tr>
                <tr>
                  <td>GLORIA</td>
                  <td>0.307</td>
                  <td>0.027</td>
                  <td>0.371</td>
                  <td>0.197</td>
                  <td>0.166</td>
                  <td>9%</td>
                </tr>
                <tr>
                  <td>MARE</td>
                  <td>0.312</td>
                  <td>0.028</td>
                  <td>0.375</td>
                  <td>0.239</td>
                  <td>0.117</td>
                  <td>10%</td>
                </tr>
                <tr>
                  <td>DUCATO</td>
                  <td>0.315</td>
                  <td>0.028</td>
                  <td>0.352</td>
                  <td>0.212</td>
                  <td>0.148</td>
                  <td>9%</td>
                </tr>
                <tr>
                  <td>CL26</td>
                  <td>0.319</td>
                  <td>0.038</td>
                  <td>0.407</td>
                  <td>0.188</td>
                  <td>0.224</td>
                  <td>12%</td>
                </tr>
                <tr>
                  <td>TERRA</td>
                  <td>0.321</td>
                  <td>0.031</td>
                  <td>0.387</td>
                  <td>0.199</td>
                  <td>0.219</td>
                  <td>10%</td>
                </tr>
                <tr>
                  <td>KRISTALL</td>
                  <td>0.325</td>
                  <td>0.028</td>
                  <td>0.380</td>
                  <td>0.221</td>
                  <td>0.159</td>
                  <td>9%</td>
                </tr>
                <tr>
                  <td>DARDO</td>
                  <td>0.333</td>
                  <td>0.036</td>
                  <td>0.409</td>
                  <td>0.197</td>
                  <td>0.192</td>
                  <td>11%</td>
                </tr>
                <tr>
                  <td>SIRIO_CL</td>
                  <td>0.333</td>
                  <td>0.035</td>
                  <td>0.419</td>
                  <td>0.193</td>
                  <td>0.199</td>
                  <td>11%</td>
                </tr>
                <tr>
                  <td>SOLE</td>
                  <td>0.334</td>
                  <td>0.028</td>
                  <td>0.403</td>
                  <td>0.272</td>
                  <td>0.116</td>
                  <td>9%</td>
                </tr>
                <tr>
                  <td>SELENIO</td>
                  <td>0.336</td>
                  <td>0.109</td>
                  <td>0.397</td>
                  <td>0.223</td>
                  <td>0.189</td>
                  <td>31%</td>
                </tr>
                <tr>
                  <td>CENTAURO</td>
                  <td>0.346</td>
                  <td>0.034</td>
                  <td>0.431</td>
                  <td>0.178</td>
                  <td>0.206</td>
                  <td>10%</td>
                </tr>
                <tr>
                  <td>Prob.</td>
                  <td>&lt;.0001</td>
                  <td>&lt;.0001</td>
                  <td>0.0008</td>
                  <td>0.0084</td>
                  <td>0.1262</td>
                  <td>&lt;.0001</td>
                </tr>
              </tbody>
            </table>
          </table-wrap>
        </sec>
        <sec id="idm1842355900">
          <title>Nitrogen – Yield Correlations.  </title>
          <p>As shown in <xref ref-type="fig" rid="idm1842300468">Figure 9</xref>, the N-pre-PI and the NDRE in the sample field, which is composed of 311 plots, are not related. In that year (2018), the N-pre-PI  was distributed twice according to the 2017 production maps. Instead, the N-PI was administered in a strong negative correlation with the NDRE of each plot                      (r –0.92; <xref ref-type="table" rid="idm1842298380">Table 6</xref>), and is clearly visible in <xref ref-type="fig" rid="idm1842299460">Figure 10</xref>. However, the Yield was not related to N-pre-PI (-0.19), but was negatively related to N-PI (-0.58) and N-tot               (-0,38), while it was overall positive for NDRE (R<xref ref-type="bibr" rid="ridm1843295444">2</xref>= 0.29, <xref ref-type="fig" rid="idm1842560660">Figure 7</xref>; r=0.54 <xref ref-type="table" rid="idm1842298380">Table 6</xref>). </p>
          <fig id="idm1842300468">
            <label>Figure 9.</label>
            <caption>
              <title> A -null-relationship example between NDRE and N pre-pi.</title>
            </caption>
            <graphic xlink:href="images/image9.jpg" mime-subtype="jpg"/>
          </fig>
          <fig id="idm1842299460">
            <label>Figure 10.</label>
            <caption>
              <title> A strong negative regression of N-PI on the NDRE value, and a mild positive relationship between NDRE and Yield.</title>
            </caption>
            <graphic xlink:href="images/image10.jpg" mime-subtype="jpg"/>
          </fig>
          <table-wrap id="idm1842298380">
            <label>Table 6.</label>
            <caption>
              <title> Input / Output means, correlation and partial correlation from a field over a two-year period.</title>
            </caption>
            <table rules="all" frame="box">
              <tbody>
                <tr>
                  <td>Items</td>
                  <td>Means</td>
                  <td>St. dev.</td>
                  <td>Coef. Var</td>
                  <td colspan="4">Pearson Correlations</td>
                </tr>
                <tr>
                  <td>2017</td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td>N-0</td>
                  <td>N-PI</td>
                  <td>N-Tot</td>
                  <td>NDRE</td>
                </tr>
                <tr>
                  <td>Yield</td>
                  <td>9.5</td>
                  <td>0.753</td>
                  <td>7.9%</td>
                  <td>0.02</td>
                  <td>-0.11</td>
                  <td>-0.04</td>
                  <td>0.14</td>
                </tr>
                <tr>
                  <td>NDRE</td>
                  <td>0.318</td>
                  <td>0.026</td>
                  <td>8%</td>
                  <td>0.23</td>
                  <td>-0.93</td>
                  <td>-0.25</td>
                  <td>1</td>
                </tr>
                <tr>
                  <td>N-pi</td>
                  <td>63</td>
                  <td>6.9</td>
                  <td>11%</td>
                  <td> </td>
                  <td>1</td>
                  <td>0.30</td>
                  <td> </td>
                </tr>
                <tr>
                  <td>N-0</td>
                  <td>143</td>
                  <td>13.1</td>
                  <td>9%</td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                </tr>
                <tr>
                  <td>N-tot</td>
                  <td>206</td>
                  <td>13.4</td>
                  <td>7%</td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                </tr>
                <tr>
                  <td>N-pi/N-0</td>
                  <td>30%</td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                </tr>
                <tr>
                  <td>NUE</td>
                  <td>21.70</td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                </tr>
                <tr>
                  <td>Yield,N .NDRE</td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td>0.06</td>
                  <td>-0.01</td>
                  <td> </td>
                </tr>
                <tr>
                  <td>2018</td>
                  <td>Yield</td>
                  <td> </td>
                  <td> </td>
                  <td>N-0</td>
                  <td>N-pi</td>
                  <td>N-tot</td>
                  <td>NDRE</td>
                </tr>
                <tr>
                  <td>Yield</td>
                  <td>8.7</td>
                  <td>1.013</td>
                  <td>11.6%</td>
                  <td>-0.19</td>
                  <td>-0.58</td>
                  <td>-0.38</td>
                  <td>0.54</td>
                </tr>
                <tr>
                  <td>NDRE</td>
                  <td>0.319</td>
                  <td>0.045</td>
                  <td>14%</td>
                  <td>-0.16</td>
                  <td>-0.92</td>
                  <td>-0.47</td>
                  <td>1</td>
                </tr>
                <tr>
                  <td>N-pi</td>
                  <td>47</td>
                  <td>8.7</td>
                  <td>19%</td>
                  <td> </td>
                  <td>1</td>
                  <td>0.50</td>
                  <td> </td>
                </tr>
                <tr>
                  <td>N-0</td>
                  <td>118</td>
                  <td>20.7</td>
                  <td>18%</td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td>
                    <bold> </bold>
                  </td>
                </tr>
                <tr>
                  <td>N-tot</td>
                  <td>164</td>
                  <td>23.7</td>
                  <td>14%</td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                </tr>
                <tr>
                  <td>N-pi/N-0</td>
                  <td>28%</td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                </tr>
                <tr>
                  <td>NUE</td>
                  <td>18.85</td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                </tr>
                <tr>
                  <td>Yield,N .NDRE</td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td> </td>
                  <td>-0.22</td>
                  <td>-0.17</td>
                  <td>
                    <bold> </bold>
                  </td>
                </tr>
              </tbody>
            </table>
            <table-wrap-foot>
              <fn id="idm1842291620">
                <label/>
                <p>Table 6. summarizes the O/I relationships for two years. The N-PI was about 28-30% of the total. The yield-N-tot correlation, which was negative for both years, became less negative at NDRE parity.</p>
              </fn>
            </table-wrap-foot>
          </table-wrap>
          <p>In <xref ref-type="fig" rid="idm1842177964">Figure 11</xref>, a parabolic regression of the yield on N-tot (in violet) shows that a maximum yield-zone was attained. Nevertheless, the points in red below the trajectory indicate sparse areas of nitrogen inefficiency.</p>
          <fig id="idm1842177964">
            <label>Figure 11.</label>
            <caption>
              <title> Regression of the Yield on N-pi and N-total and regression of the NDRE on the N-total.</title>
            </caption>
            <graphic xlink:href="images/image11.jpg" mime-subtype="jpg"/>
          </fig>
        </sec>
      </sec>
    </sec>
    <sec id="idm1842291116" sec-type="discussion">
      <title>Discussion</title>
      <sec id="idm1842290756">
        <title>Repeatability Model.</title>
        <p>The first lesson learned from the farm was that there was a low repeatability of parcel yields in subsequent years. Since this parameter is independent of the operators, it is generally neglected. The PA capabilities can now be used to monitor the parcel responses at a capillary level, thus parcel repeatability could be considered by means of algorithms, especially considering that its positive evolution seems to follow the increase in variability. In fact, a perfect PA aims to a strong homogeneity in parcel production, ergo a                zero-correlation between the O/I framework. The germinability of the seeds for flooded rice production is a very critical point, and a variable steam density may affect the yield <xref ref-type="bibr" rid="ridm1843401636">4</xref>,<xref ref-type="bibr" rid="ridm1843158372">5</xref></p>
      </sec>
      <sec id="idm1842289676">
        <title>Variability Increase.</title>
        <p>The second lesson pertained to the increase in variability of the yield and, to a lesser extent, in NDRE after that a strong negative correlation (&lt; -0.90) was imposed on the N-pi variable fertilization, as inversely proportional to the vegetative vigour attained near PI. Cordero et al <xref ref-type="bibr" rid="ridm1843144364">8</xref> in a two-year study on experimental plots fertilized at 0÷300 N-tot, have recently proposed a more refined but similar inverse function to establish the acceptable reduction relative to the maximum obtainable yield.  In a study on sugarcane <xref ref-type="bibr" rid="ridm1843142132">9</xref>, the biomass was the main crop parameter that influenced canopy sensor readings, because biomass readings generally result in stalk yields. Thus, canopy sensor readings can efficiently predict relative sugarcane yields when data that are normalized to the mean of the field are considered. Hence, an algorithm that considers this relationship was established, based on an r -0.68 correlation between NDRE and its coefficient of variation, which was lower than the -0.33 of the present work. However, the NDRE in the 16 sugarcane plots was positively correlated with the yield (+0.82), so that the N topdressing was calibrated in positive <italic>vs.</italic> NDRE, and not in negative as in the present work. Thus an increase in NDRE should reduce the yield variability, but this has not yet been observed in real fields.</p>
        <p>The improvement of the mean and maximum yields, and not of the minimum, depends on several plots where the limiting factor cannot be referred to the N availability. A next challenge will be to check the repeatability for yield in those critical plots and to find which is the limiting factor, or, more simply, to reduce the N rate, avoiding a waste. </p>
      </sec>
      <sec id="idm1842288740">
        <title>N Correlations.</title>
        <p>The third lesson concerned the negative correlation between N-tot and yield, which resulted even after absorbing the N-pi in the N-tot. In the  experimental plot studies, the correlations were generally positive, and even very high, until the maximum yield was reached <xref ref-type="bibr" rid="ridm1843158372">5</xref>,<xref ref-type="bibr" rid="ridm1843144364">8</xref> but results from the real fields are much less optimistic: i.e. Zavattaro et al <xref ref-type="bibr" rid="ridm1843147532">10</xref>  observed a sparse regression (+0.0125 t kgN-tot<sup>-1</sup>) of the yield (avg. 6.55 t ha<sup>-1</sup>) on the N-tot (121.6 kg ha<sup>-1</sup>) for seven variety groups from 67 land units in NW Italy, where the correlation coefficient was recalculated to r  0.26; the average NUE, compared to the present work, was 54 kg kg<sup>-1</sup>,that is,an increased value of 15%, while the N-tot and yield appeared reduced by 27% and 16%, respectively.</p>
      </sec>
      <sec id="idm1842286868">
        <title>A local relativity.</title>
        <p>The fourth lesson is related the uncertainty of the reference parcels which identified at zero- input <xref ref-type="bibr" rid="ridm1843133716">11</xref>, or at well-fertilized input<xref ref-type="bibr" rid="ridm1843158372">5</xref> or by a corrective sufficiency index <xref ref-type="bibr" rid="ridm1843144364">8</xref>,<xref ref-type="bibr" rid="ridm1843158372">5</xref>, which are necessary to set up the production models and the PA. As shown in <xref ref-type="fig" rid="idm1842300468">Figure 9</xref>, the NDRE response over different N-pre-pi was highly variable, that weakens the right point of reference.</p>
        <p>The NDRE variability also depends to a great extent on the cultivar, and on the evolution of the temperature  and  light intensity of the growing season, and  it is therefore not automatic to apply the correlation between NDRE and the N rate for PI fertilization of the previous years. The empirical method applied till now on the farm is:  i) to consider the prescription threshold  table applied in the same cultivar in the previous years; ii)  to detect by a drone the gross average NDRE for this cultivar in the current year, and iii)  to adapt the prescription threshold table to this parameter. It could also be useful to adapt the N rates to the weather forecast for the following 40 days, if it could be considered reliable.  </p>
      </sec>
    </sec>
    <sec id="idm1842285428" sec-type="conclusions">
      <title>Conclusions</title>
      <p>The study is detached from the usual basic models and applications of the PA, but unfortunately there are no detailed references of similar duration and width to be compared. However, PA is about, and interesting lessons, derived from real and not from model domain, featured the evolution of high production rice fields. The PA on this farm increased the yield for the same total input of N, but also its variability was increased – and not reduced, as predicted by the             theory – but  strengthening the repeatability, a neglected parameter.</p>
      <p>The present elaborations of field parcel data suggest that the actual applied N rates are near, or over, the maximum of the parabolic curve that represents the non-linear correlation between  N-tot and Yield. Slightly reducing the maximum N rates would probably not affect the maximum yields.  </p>
      <p>In order to reduce the variability of NDRE and of the yields, the first factor that must be considered is the plant density: having an even density of between 200 and 250 plants m<sup>-2</sup> should be the key to improving the results (<xref ref-type="fig" rid="idm1842166516">Figure 12</xref>)</p>
      <fig id="idm1842166516">
        <label>Figure 12.</label>
        <caption>
          <title> An example of high variability in the            number of plants (up) and panicles (down) per square meter, manually measured in the same sites, with the help of a GPS portable device.</title>
        </caption>
        <graphic xlink:href="images/image12.jpeg" mime-subtype="jpeg"/>
      </fig>
      <p>An advantage of the reduction in the N rates at PI, where NDRE is already high, is a reduction in  the risk of  attacks of <italic>Pyricularia</italic><italic>grisea</italic>, a fungal disease that is increased by N excesses. This advantage is very complex  to be estimated in a farm environment.</p>
      <p>All over the paper we have deliberately avoided representing maps of vigor and minute details of production and N-supply. The fact that the repeatability was barely +0.31 over 6 years indicates that the soil factor &amp; N-pre-pi explains less than 10% of the variability, so we would have shown a 90% noise in the maps</p>
      <p>This paper underlines that PA still has a huge potential that still must be explored.</p>
    </sec>
    <sec id="idm1842252620">
      <title>Acknowledgments</title>
      <p>The authors wish to thank the Fondazione CRT, Torino – Italy, for the financial support to the scientific activities of the “Accademia di Agricoltura di Torino”. </p>
    </sec>
  </body>
  <back>
    <ref-list>
      <ref id="ridm1843298828">
        <label>1.</label>
        <mixed-citation xlink:type="simple" publication-type="journal">
          <name>
            <surname>Confalonieri</surname>
            <given-names>R</given-names>
          </name>
          <name>
            <surname>Bocchi</surname>
            <given-names>S</given-names>
          </name>
          <article-title>Evaluation of CropSyst for simulating the yield of flooded rice in northern Italy.European journal of agronomy23</article-title>
          <date>
            <year>2005</year>
          </date>
          <fpage>315</fpage>
          <lpage>326</lpage>
        </mixed-citation>
      </ref>
      <ref id="ridm1843295444">
        <label>2.</label>
        <mixed-citation xlink:type="simple" publication-type="journal">
          <name>
            <surname>Balasubramanian</surname>
            <given-names>V</given-names>
          </name>
          <name>
            <surname>Alves</surname>
            <given-names>B</given-names>
          </name>
          <name>
            <surname>Aulak</surname>
            <given-names>M</given-names>
          </name>
          <name>
            <surname>Bekunda</surname>
            <given-names>M</given-names>
          </name>
          <name>
            <surname>Cai</surname>
            <given-names>Z</given-names>
          </name>
          <name>
            <surname>Drinkwater</surname>
            <given-names>L</given-names>
          </name>
          <name>
            <surname>Oenema</surname>
            <given-names>O</given-names>
          </name>
          <article-title>Crop, environmental,and management factors affecting nitrogen use efficiency</article-title>
          <date>
            <year>2004</year>
          </date>
          <source>Agriculture and the Nitrogen Cycle,edited by Mosier AR, Syers J K, Freney J,SCOPE</source>
          <volume>65</volume>
          <fpage>19</fpage>
          <lpage>33</lpage>
        </mixed-citation>
      </ref>
      <ref id="ridm1843303484">
        <label>3.</label>
        <mixed-citation xlink:type="simple" publication-type="journal">
          <name>
            <surname>Brisson</surname>
            <given-names>N</given-names>
          </name>
          <name>
            <surname>Gary</surname>
            <given-names>C</given-names>
          </name>
          <name>
            <surname>Justes</surname>
            <given-names>E</given-names>
          </name>
          <name>
            <surname>Roche</surname>
            <given-names>R</given-names>
          </name>
          <name>
            <surname>Mary</surname>
            <given-names>B</given-names>
          </name>
          <name>
            <surname>Ripoche</surname>
            <given-names>D</given-names>
          </name>
          <article-title>An overview of the crop model STICS</article-title>
          <date>
            <year>2003</year>
          </date>
          <source>European Journal of agronomy</source>
          <volume>18</volume>
          <fpage>309</fpage>
          <lpage>332</lpage>
        </mixed-citation>
      </ref>
      <ref id="ridm1843401636">
        <label>4.</label>
        <mixed-citation xlink:type="simple" publication-type="journal">
          <name>
            <surname>Hirzel</surname>
            <given-names>J</given-names>
          </name>
          <name>
            <surname>Pedreros</surname>
            <given-names>A</given-names>
          </name>
          <name>
            <surname>Cordero</surname>
            <given-names>K</given-names>
          </name>
          <article-title>Effect of nitrogen rates and split nitrogen fertilization on grain yield and its components in flooded rice.Chilean</article-title>
          <date>
            <year>2011</year>
          </date>
          <source>Journal of Agricultural Research,71</source>
          <fpage>437</fpage>
        </mixed-citation>
      </ref>
      <ref id="ridm1843158372">
        <label>5.</label>
        <mixed-citation xlink:type="simple" publication-type="book">
          <name>
            <surname>Xue</surname>
            <given-names>L</given-names>
          </name>
          <name>
            <surname>Li</surname>
            <given-names>G</given-names>
          </name>
          <name>
            <surname>Qin</surname>
            <given-names>X</given-names>
          </name>
          <name>
            <surname>Yang</surname>
            <given-names>L</given-names>
          </name>
          <name>
            <surname>Zhang</surname>
            <given-names>H</given-names>
          </name>
          <article-title>Topdressing nitrogen recommendation for early rice with an active sensor in south China.Precision</article-title>
          <date>
            <year>2014</year>
          </date>
          <chapter-title>Agriculture,15</chapter-title>
          <fpage>95</fpage>
          <lpage>110</lpage>
        </mixed-citation>
      </ref>
      <ref id="ridm1843157364">
        <label>6.</label>
        <mixed-citation xlink:type="simple" publication-type="book">
          <name>
            <surname>Pallottino</surname>
            <given-names>F</given-names>
          </name>
          <name>
            <surname>Biocca</surname>
            <given-names>M</given-names>
          </name>
          <name>
            <surname>Nardi</surname>
            <given-names>P</given-names>
          </name>
          <name>
            <surname>Figorilli</surname>
            <given-names>S</given-names>
          </name>
          <name>
            <surname>Menesatti</surname>
            <given-names>P</given-names>
          </name>
          <name>
            <surname>Costa</surname>
            <given-names>C</given-names>
          </name>
          <article-title>Science mapping approach to analyze the research evolution on precision agriculture: World</article-title>
          <date>
            <year>2018</year>
          </date>
          <chapter-title>EU and Italian situation.Precision Agriculture,19</chapter-title>
          <fpage>1011</fpage>
          <lpage>1026</lpage>
        </mixed-citation>
      </ref>
      <ref id="ridm1843160532">
        <label>7.</label>
        <mixed-citation xlink:type="simple" publication-type="journal"><name><surname>ISTAT</surname><given-names/></name><article-title>Superficie (ettari) e produzione (quintali)</article-title><date><year>2018</year></date>
http://agri.istat.it/jsp/dawinci.jsp?q=plC020000010000011000&amp;an=2013&amp;ig=1&amp;ct=244&amp;id=15A|18A|25A


</mixed-citation>
      </ref>
      <ref id="ridm1843144364">
        <label>8.</label>
        <mixed-citation xlink:type="simple" publication-type="journal">
          <name>
            <surname>Cordero</surname>
            <given-names>E</given-names>
          </name>
          <name>
            <surname>Moretti</surname>
            <given-names>B</given-names>
          </name>
          <name>
            <surname>E</surname>
            <given-names>F Miniotti</given-names>
          </name>
          <name>
            <surname>Tenni</surname>
            <given-names>D</given-names>
          </name>
          <name>
            <surname>Beltarre</surname>
            <given-names>G</given-names>
          </name>
          <name>
            <surname>Romani</surname>
            <given-names>M</given-names>
          </name>
          <name>
            <surname>Sacco</surname>
            <given-names>D</given-names>
          </name>
          <article-title>Fertilisation strategy and ground sensor measurements to optimise rice yield</article-title>
          <date>
            <year>2018</year>
          </date>
          <source>European Journal</source>
          <volume>99</volume>
          <fpage>177</fpage>
          <lpage>185</lpage>
        </mixed-citation>
      </ref>
      <ref id="ridm1843142132">
        <label>9.</label>
        <mixed-citation xlink:type="simple" publication-type="journal">
          <name>
            <surname>L</surname>
            <given-names>R Amaral</given-names>
          </name>
          <name>
            <surname>J</surname>
            <given-names>P Molin</given-names>
          </name>
          <name>
            <surname>J</surname>
            <given-names>S Schepers</given-names>
          </name>
          <article-title>Algorithm for variable-rate nitrogen application in sugarcane based on active crop canopy sensor.Agronomy</article-title>
          <date>
            <year>2015</year>
          </date>
          <source>Journal</source>
          <volume>107</volume>
          <fpage>1513</fpage>
          <lpage>1523</lpage>
        </mixed-citation>
      </ref>
      <ref id="ridm1843147532">
        <label>10.</label>
        <mixed-citation xlink:type="simple" publication-type="book">
          <name>
            <surname>Zavattaro</surname>
            <given-names>L</given-names>
          </name>
          <name>
            <surname>Romani</surname>
            <given-names>M</given-names>
          </name>
          <name>
            <surname>Sacco</surname>
            <given-names>D</given-names>
          </name>
          <name>
            <surname>Bassanino</surname>
            <given-names>M</given-names>
          </name>
          <name>
            <surname>Grignani</surname>
            <given-names>C</given-names>
          </name>
          <article-title>Fertilization management of paddy fields</article-title>
          <date>
            <year>2008</year>
          </date>
          <chapter-title>in Piedmont (NW Italy).Italian Journal of Agronomy,3</chapter-title>
          <fpage>201</fpage>
          <lpage>212</lpage>
        </mixed-citation>
      </ref>
      <ref id="ridm1843133716">
        <label>11.</label>
        <mixed-citation xlink:type="simple" publication-type="journal">
          <name>
            <surname>R</surname>
            <given-names>W Mullen</given-names>
          </name>
          <name>
            <surname>K</surname>
            <given-names>W Freeman</given-names>
          </name>
          <name>
            <surname>W</surname>
            <given-names>R Raun</given-names>
          </name>
          <name>
            <surname>G</surname>
            <given-names>V Johnson</given-names>
          </name>
          <name>
            <surname>M</surname>
            <given-names>L Stone</given-names>
          </name>
          <name>
            <surname>J</surname>
            <given-names>B Solie</given-names>
          </name>
          <article-title>Identifying an in-season response index and the potential to increase wheat yield with nitrogen</article-title>
          <date>
            <year>2003</year>
          </date>
          <source>Agron J</source>
          <volume>95</volume>
          <fpage>347</fpage>
          <lpage>351</lpage>
        </mixed-citation>
      </ref>
    </ref-list>
  </back>
</article>
